Lectin from Chromobacterium violaceum - role of water molecule in the carbohydrate binding site

DEJMKOVÁ, Eva, Martina POKORNÁ, Jan ALÁN and Michaela WIMMEROVÁ. Lectin from Chromobacterium violaceum - role of water molecule in the carbohydrate binding site. In XIV. Setkání biochemiků a molekulárních biologů. 2010. ISBN 978-80-210-5164-5.

Basic information

• Original name: Lectin from Chromobacterium violaceum - role of water molecule in the carbohydrate binding site
• Name in Czech: Lektin z Chromobacterium violaceum - role molekuly vody ve vazebném místě pro sacharid
• Authors: DEJMKOVÁ, Eva (203 Czech Republic, belonging to the institution), Martina POKORNÁ (203 Czech Republic, belonging to the institution), Jan ALÁN (203 Czech Republic, belonging to the institution) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: XIV. Setkání biochemiků a molekulárních biologů, 2010.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/10:00044124
• Organization unit: Faculty of Science
• ISBN: 978-80-210-5164-5
• Keywords (in Czech): Lektiny; Chromobacterium violaceum; role molekuly vody
• Keywords in English: Lectin; Chromobacterium violaceum; role of a water molecule

Abstract

CV-IIL is a lectin from human opprotunistic pathogen microorganism Chromobacterium violaceum. Crystal structure of this lectin demonstrates, that there is one water molecule in the carbohydrate binding site, which plays a special role by bridging sugar with backbone nitrogen atom and also with side chain of the amino acid threonine in position 97. Substitution of this amino acid should lead to changes of thermodynamical parametres (an enthalpic and entropic contribution to the free energy of the interaction lectin-sugar) that could be determine by an isothermal titration microcalorimetry method.

Abstract (in Czech)

CV-IIL je lektin z patogenního organismu Chromobacterium violaceum. Krystalová struktura proteinu ukazuje, že ve vazebném místě pro sacharid je přítomna jedna molekula vody, která zprostředkovává vazbu monosacharidu s aminokyselinou threonin v pozici 97. Záměna této aminokyseliny může vést ke změnám termodynamických parametrů interakce lektin-sacharid (změny entalpie a entropie), které mohou být určeny pomocí metody isotermální titrační mikrokalorimatrie.

Links

• MSM0021622413, plan (intention): Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment