Oligonucleotide array-based comparative genomic hybridization analyses of gain 1q21 in multiple myeloma

ZAORALOVÁ, Romana, Pavel NĚMEC, Henrieta GREŠLIKOVÁ, Jan SMETANA, Lucie KOVÁŘOVÁ, Roman HÁJEK and Petr KUGLÍK. Oligonucleotide array-based comparative genomic hybridization analyses of gain 1q21 in multiple myeloma. In European Human Genetics Conference. 2010.

Basic information

• Original name: Oligonucleotide array-based comparative genomic hybridization analyses of gain 1q21 in multiple myeloma
• Name in Czech: Oligonukleotide array-CGH analýza zisku 1q21 u mnohočetného myelomu
• Authors: ZAORALOVÁ, Romana, Pavel NĚMEC, Henrieta GREŠLIKOVÁ, Jan SMETANA, Lucie KOVÁŘOVÁ, Roman HÁJEK and Petr KUGLÍK.
• Edition: European Human Genetics Conference, 2010.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 30200 3.2 Clinical medicine
• Country of publisher: Sweden
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Medicine
• Keywords (in Czech): Oligonukleotide array-CGH; zisk 1q21; mnohočetný myelom
• Keywords in English: Oligonukleotide array-CGH; gain 1q21; multiple myeloma

Abstract

Using oligonucleotide array-CGH we analyzed 41 MM samples with gain of 1q21 (CKS1B gene) detected previously by FISH. As input material for array-CGH we used DNA from CD138+ cells separated from bone marrow using magnetic or fluorescence activated cell separation. Taking together 76% (13 of 41) cases had gained not only 1q21 locus but whole 1q arm. Moreover, another 17% (7 of 41) had at least 100 MB gain anywhere at the 1q arm. It is obvious, that not only CKS1B gene, but other genes are affected as well, for example IL-6R or BCL6. Many of them are possibly negative prognostic factors in MM. According to the FISH data (in about 95% cases we detect max. two extra copies of CKS1B), we did not find any high-level amplification of 1q21. In all cases, 1q aberrations were associated with additional copy number alterations, including monosomy of chromosome 13 or hyperdiploidy.

Abstract (in Czech)

Pomocí oligonucleotidové array-CGH bylo analyzováno 41 pacientů s mnohočetným myelomem, u nichž byl pomocí FISH nalezen zisk 1q21. Pro analýzu byla využita DNA ze separovaných CD138+ buněk. Celkem u 13 z 41 pacientů nebyl nalezen pouze zisk 1q21, ale zisk celého 1q raménka - nejen změna v genu CKS1B, ale i IL-6R a BCL6, což jsou popsané možné negativní prognostické faktory.

Links

• LC06027, research and development project: Name: Univerzitní výzkumné centrum - Česká myelomová skupina (Acronym: LC MGUS)

Investor: Ministry of Education, Youth and Sports of the CR, University Research Centre - Czech Myeloma Group

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

• MSM0021622434, plan (intention): Name: Od klasických prognostických markerů ke klinicky aplikovatelným farmakogenomickým a farmakoproteomickým projektům u mnohočetného myelomu a monoklonálních gamapatií

Investor: Ministry of Education, Youth and Sports of the CR, From classic prognostic markers to clinical applications in selected pharmacogenomic and pharmacoproteomic projects in multiple myeloma and monoclonal gammapathies

• NS10207, research and development project: Name: Úloha abnormalit chromozómu 1 a kaskády NF-kappaB v patogenezi mnohočetného myelomu

Investor: Ministry of Health of the CR

• NS10406, research and development project: Name: Vytvoření prognostického panelu u pacientů s monoklonální gamapatií nejasného významu s cílem zabránění transformace v maligní onemocnění.

Investor: Ministry of Health of the CR