MALDI-MS profiling of lactic acid bacteria
ŠEDO, Ondrej, Andrea TESHIM, Marta DUŠKOVÁ, Magdaléna KAČALOVÁ, Renata KARPÍŠKOVÁ, Ivo SEDLÁČEK and Zbyněk ZDRÁHAL. MALDI-MS profiling of lactic acid bacteria. In Proceedings of the International Scientific Conference on Probiotics and Prebiotics. 2010. ISBN 978-80-970168-4-5. |
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Basic information | |
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Original name | MALDI-MS profiling of lactic acid bacteria |
Name in Czech | MALDI-MS profilování bakterií mléčného kvašení |
Authors | ŠEDO, Ondrej, Andrea TESHIM, Marta DUŠKOVÁ, Magdaléna KAČALOVÁ, Renata KARPÍŠKOVÁ, Ivo SEDLÁČEK and Zbyněk ZDRÁHAL. |
Edition | Proceedings of the International Scientific Conference on Probiotics and Prebiotics, 2010. |
Other information | |
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Original language | English |
Type of outcome | Conference abstract |
Field of Study | 10600 1.6 Biological sciences |
Country of publisher | Slovakia |
Confidentiality degree | is not subject to a state or trade secret |
Organization unit | Faculty of Science |
ISBN | 978-80-970168-4-5 |
Keywords (in Czech) | MALDI-TOF MS profilování; bakterie mléčného kvašení; identifikace |
Keywords in English | MALDI-TOF MS profiling; lactic acid bacteria; identification |
Tags | International impact |
Changed by | Changed by: Mgr. Ondrej Šedo, Ph.D., učo 13589. Changed: 10/12/2010 10:19. |
Abstract |
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Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) is a well established method for peptide and protein molecular weight determination routinely applied mostly in proteomic research. Due to its recently described capability to provide reproducible fingerprint spectra of whole bacterial cells or extracts, the method is now increasing its share also in the field of bacteria identification and taxonomy. The unique advantages of the method concern above all its rapidity and discriminatory power. The aim of this work was to examine the robustness and discriminatory power of MALDI-TOF MS profiling on a large set of lactic acid bacteria isolated from diverse sources. |
Abstract (in Czech) |
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MALDI-TOF MS je zavedenou metodou pro stanovení molekulové hmotnosti proteinů a peptidů v proteomickém výzkumu. V poslední době začíná na základě své schopnosti poskytovat reprodukovatelné fingerprinty být aplikována i v mikrobiologii pro identifikcai a klasifikaci mikroorganismů. Cílem studie bylo zhodnocení použitelnosti metody v oblasti identifikace bakterií mléčného kvašení. |
Links | |
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LC06034, research and development project | Name: Regulace morfogeneze rostlinných buněk a orgánů |
Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs | |
MSM0021622415, plan (intention) | Name: Molekulární podstata buněčných a tkáňových regulací |
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations | |
MSM0021622416, plan (intention) | Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase |
Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time | |
MSM6215712402, plan (intention) | Name: Veterinární aspekty bezpečnosti a kvality potravin |
2B08068, research and development project | Name: Výzkum možností aplikace automatizovaných a poloautomatizovaných metod pro identifikaci a charakterizaci nových kmenů baktérií s probiotickými vlastnostmi s cílem rozšíření specifických genetických zdrojů pro prevenci civilizačních onemocnění. (Acronym: GASTROPROBIO) |
Investor: Ministry of Education, Youth and Sports of the CR, Research into possibilities of application both automated and semi-automated methods for identification and describing new microorganisms with probiotic properties with aim to extend specific genetic sources for civilizing diseases prevention |
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