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@article{890012,
author = {Křížková, Soňa and Húska, Dalibor and Beklová, Miroslava and Hubálek, Jaromír and Adam, Vojtěch and Trnková, Libuše and Kizek, René},
article_number = {3},
keywords = {Silver; Biosensors; Voltammetry; Interaction},
language = {eng},
issn = {0730-7268},
journal = {ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY},
title = {PROTEIN-BASED ELECTROCHEMICAL BIOSENSOR FOR DETECTION OF SILVER(I) IONS},
volume = {29/2010},
year = {2010}
}
TY - JOUR
ID - 890012
AU - Křížková, Soňa - Húska, Dalibor - Beklová, Miroslava - Hubálek, Jaromír - Adam, Vojtěch - Trnková, Libuše - Kizek, René
PY - 2010
TI - PROTEIN-BASED ELECTROCHEMICAL BIOSENSOR FOR DETECTION OF SILVER(I) IONS
JF - ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
VL - 29/2010
IS - 3
SN - 07307268
KW - Silver
KW - Biosensors
KW - Voltammetry
KW - Interaction
N2 - Silver(I) ions are extremely toxic to aquatic animals. Hence, monitoring of these ions in the environment is needed. The aim of the present study was to suggest a simple biosensor for silver(I) ions detection. The suggested biosensor is based on the modification of a hanging mercury drop electrode (HMDE) by the heavy metal binding protein metallothionein (MT) for silver(I) ions detection. Metallothionein accumulated for 120 s onto the HMDE surface. After rinsing the electrode, the biosensor (MT modified HMDE) was prepared prior to detection of silver(I) ions. The biosensor was immersed in a solution containing silver(I) ions. These ions were bound to the MT structure. Furthermore, the electrode was rinsed and transferred to a pure supporting electrolyte solution, in which no interference was present. Under these experimental conditions, other signals relating to heavy metals naturally occurring in MT were not detected. This phenomenon confirms the strong affinity of silver(I) ions for MT. The suggested biosensor responded well to higher silver(I) ion concentrations. The relative standard deviation for measurements of concentrations higher than 50 mu M was approximately 2% (n = 8). In the case of concentrations lower than 10 mu M, the relative standard deviation increased to 10% (n = 8). The detection limit (3 signal/noise) for silver(I) ions was estimated as 500 nM.
ER -
KŘÍŽKOVÁ, Soňa, Dalibor HÚSKA, Miroslava BEKLOVÁ, Jaromír HUBÁLEK, Vojtěch ADAM, Libuše TRNKOVÁ a René KIZEK. PROTEIN-BASED ELECTROCHEMICAL BIOSENSOR FOR DETECTION OF SILVER(I) IONS. \textit{ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY}. 2010, roč.~29/2010, č.~3, 5 s. ISSN~0730-7268.
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