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@proceedings{890213,
author = {Kadeřávek, Pavel and Diehl, Carl and Motáčková, Veronika and Šanderová, Hana and Žídek, Lukáš and Krásný, Libor and Sklenář, Vladimír and Akke, Mikael},
booktitle = {8th Discussions in Structural Molecular Biology Nove Hrady, 2010},
title = {ANALYSIS OF BACKBONE MOTIONS OF DELTA SUBUNIT OF RNA POLYMERASE FROM BACILLUS SUBTILIS},
url = {http://www.structbio.eu/Current_Meeting.html},
year = {2010}
}
TY - CONF
ID - 890213
AU - Kadeřávek, Pavel - Diehl, Carl - Motáčková, Veronika - Šanderová, Hana - Žídek, Lukáš - Krásný, Libor - Sklenář, Vladimír - Akke, Mikael
PY - 2010
TI - ANALYSIS OF BACKBONE MOTIONS OF DELTA SUBUNIT OF RNA POLYMERASE FROM BACILLUS SUBTILIS
UR - http://www.structbio.eu/Current_Meeting.html
N2 - RNA polymerase plays a fundamental role in the process of protein synthesis because it is responsible for the DNA transcription. The RNA polymerase of Gram positive bacteria consists of seven subunits. The motions of delta subunit from Bacillus subtilis was investigated within this project. The previous experiments revealed the delta subunit increases the transcriptional specificity [1][2][3][4][5][6] and efficiency of RNA synthesis [4][5][6]. Delta subunit is a two domain protein. The N-terminal domain has a well defined structure while the C-terminal domain is disordered. The backbone dynamics of the N-terminal domain of delta subunit was studied to revealed the functionally important parts of the molecule. The dynamics of the system was investigated by NMR techniques. The experiments were based on the measurement of relaxation rates of backbone 15N-1H spin pair. Both the motions at fast (pico-nanoseconds) and slow (micro-miliseconds) timescales were studied. In order to explore subnanoseconds motions, the standard set of NMR experiments including R1, R2 and NOE were performed at two magnetic fields (500 MHz, 600 MHz) and the obtained data were interpreted within the Model-Free approach [7][8]. The CPMG [9][10] and T1rho [9] experiments were utilized to inspect the slow motions. The experiments and dynamic analyses were carried out at two temperatures 300 K and 280 K to obtain the temperature dependence of the dynamical parameters It was revealed that the parts of the molecule which exhibit extensive motions at the micro-miliseconds timescale correlates with the conserved residues in the sequence, expected to form an interaction surface with other subunits. On the other hand the residues which are the most flexible on the ps-ns timescale are located in another part of the protein [1] Achberger E.C., Whiteley H.R., Journal of Biological Chemistry, 256, 7424-7432,1981 [2] Achberger E.C., Tahara M., Whiteley H.R., Journal of Bacteriology, 150, 977-980, 1982 [3] Dobinson K.F., Spiegelman G.B., Biochemistry, 26, 8206-8213, 1987 [4] Juang Y.L., Helmann J.D., Journal of Molecular Biology, 239, 1-14, 1994 [5] Juang Y.L., Helmann J.D., Biochemistry, 34, 14270-14270, 1995 [6] Juang Y.L., Helmann J.D., Biochemistry, 34, 8465-8473, 1995 [7] Lipari G., Szabo A., Journal of American Chemical Society, 104, 4546-4559, 1982 [8] Lipari G., Szabo A., Journal of American Chemical Society, 104, 4559-4570, 1982 [9] Palmer A.G., Kroenke C.D., Loria J.P., Methods in Enzymology, 339, 204-238, 2001 [10]Long D., Liu M.L., Yang D.W., Journal of American Chemical Society, 130, 2432-2433, 2008
ER -
KADEŘÁVEK, Pavel, Carl DIEHL, Veronika MOTÁČKOVÁ, Hana ŠANDEROVÁ, Lukáš ŽÍDEK, Libor KRÁSNÝ, Vladimír SKLENÁŘ and Mikael AKKE. ANALYSIS OF BACKBONE MOTIONS OF DELTA SUBUNIT OF RNA POLYMERASE FROM BACILLUS SUBTILIS. In \textit{8th Discussions in Structural Molecular Biology Nove Hrady, 2010}. 2010.
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