LC-MS/MS Monitoring Human Plasma Proteins Potentionally related to Graft-versus-Host Disease

JEDLIČKOVÁ, Lenka, Jiří BRZOBOHATÝ, Jitka KABÁTHOVÁ, Jan VERNER, Šárka POSPÍŠILOVÁ and Zbyněk ZDRÁHAL. LC-MS/MS Monitoring Human Plasma Proteins Potentionally related to Graft-versus-Host Disease. 2010. ISBN 978-963-7067-20-4.

Basic information

• Original name: LC-MS/MS Monitoring Human Plasma Proteins Potentionally related to Graft-versus-Host Disease
• Name in Czech: Sledování proteinů pravděpodobně souvisejících s GVHD v lidské plazmě pomocí LC-MS/MS
• Authors: JEDLIČKOVÁ, Lenka (203 Czech Republic), Jiří BRZOBOHATÝ (203 Czech Republic), Jitka KABÁTHOVÁ (203 Czech Republic), Jan VERNER (203 Czech Republic), Šárka POSPÍŠILOVÁ (203 Czech Republic) and Zbyněk ZDRÁHAL (203 Czech Republic, guarantor).
• Edition: 2010.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 30200 3.2 Clinical medicine
• Country of publisher: Hungary
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14110/10:00040778
• Organization unit: Faculty of Medicine
• ISBN: 978-963-7067-20-4
• Keywords (in Czech): GVHD; biomarkery; LC-MS/MS
• Keywords in English: GVHD; biomarkers; LC-MS/MS
• Tags: International impact

Abstract

The aim of this study was to develop a reliable and sensitive method for determination of potential biomarkers in human plasma for early GVHD detection. Blood plasma was immunodepleted by MARS Hu-14 to remove fourteen most abundant proteins. The immunodepleted plasma samples were fractionated by LC capillary system on a reverse phase (mRP C18, Agilent) in water/acetonitrile gradient and subsequently digested with trypsin. The resulting peptides were analyzed using LC-MS/MS in autoMS/MS mode.

Abstract (in Czech)

Cílem práce bylo navrhnout a optimalizovat spolehlivou a citlivou metodu pro stanovení potencionálních biomarkerů v lidské plazmě pro časnou detekci GVHD. Plazma byla zbavena 14 nejvíce zastoupených proteinů pomocí kolonky MARS Hu-14, po té byly vzorky frakcionovány na reversní fázi na LC. Frakce byly digestovány trypsinem a analyzovány pomocí nanoLC-MS/MS.

Links

• GD204/08/H054, research and development project: Name: Molekulární mechanismy proliferace a diferenciace buněk

Investor: Czech Science Foundation, Molecular mechanisms of the cell proliferation and differentiation

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

• NS9683, research and development project: Name: Změny genomu a proteomu u leukemických pacientů s reakcí štěpu proti hostiteli (GvHD)

Investor: Ministry of Health of the CR