Determination of Metallothionein in prostate tumor cell lines

GUMULEC, Jaromír, Michal MASAŘÍK, Šárka KUCHTÍČKOVÁ, Veronika KUDLÁČKOVÁ, Michal JURAJDA, Dušan PAVLÍK, Arne ROVNÝ, Roman HRABEC, Soňa KŘÍŽKOVÁ and René KIZEK. Determination of Metallothionein in prostate tumor cell lines. In XIV. Setkání biochemiků a molekulárních biologů. 2010. ISBN 978-80-210-5164-5.

Basic information

• Original name: Determination of Metallothionein in prostate tumor cell lines
• Name in Czech: Stanovení metalothioneinu v liniích karcinomu prostaty
• Authors: GUMULEC, Jaromír, Michal MASAŘÍK, Šárka KUCHTÍČKOVÁ, Veronika KUDLÁČKOVÁ, Michal JURAJDA, Dušan PAVLÍK, Arne ROVNÝ, Roman HRABEC, Soňa KŘÍŽKOVÁ and René KIZEK.
• Edition: XIV. Setkání biochemiků a molekulárních biologů, 2010.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 30200 3.2 Clinical medicine
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Organization unit: Faculty of Medicine
• ISBN: 978-80-210-5164-5
• Keywords (in Czech): metalothionein;karcinom prostaty;nádorový marker;zinek
• Keywords in English: metalothionein;prostate cancer;tumor marker;zinc

Abstract

Prostate carcinoma (PCa) is the most common tumor and second leading cause of death due to cancer of men. Proteins such as metallothionein (MT) or alpha-methyl-CoA-racemase (AMACR) are highly overexpressed in prostate carcinoma. Althrough the role of these proteins in tumour tissue remains still unclear, it fulfills the requirements of higher specificity, which AMACR achieves at almost 100%. We have analyzed both proteins from prostate cancer cell lines LNCaP, PC-3 and 22RVL, compared to healthy prostate cell line PNT1A and then from PCa patients' serum compared to healthy serum controls. We've used immunohistochemical analysis, immunoprecipitation with magnetic nanoparticles, PAGE and dot blots. In all of these methods, we've found significant increase of expression in these samples.

Abstract (in Czech)

Metalothionein (MT) a alpha-methyl-CoA-racemáza (AMACR) jsou výrazně exprimovány u karcinomu prostaty s velkou mírou specifity pro toto onemocnění. Tyto proteiny byly analyzovány u buněčných linií LNCaP, PC-3 a 22RVL a PNT1A a ve vzorcích sér pacientů. Bylo použito imunohistochemických metod, imonoprecipitace s magnetickými nanočásticemi, SDS-PAGE a dot bloty.

Links

• GP301/09/P436, research and development project: Name: Analýza metalothioneinu u karcinomu prostaty na úrovni DNA, RNA a proteinu.

Investor: Czech Science Foundation

• NS10200, research and development project: Name: Nádorové markery, jejich stanovení a korelace s karcinomem prostaty

Investor: Ministry of Health of the CR