Determination of Metallothionein in prostate tumor cell lines

a 2010

Determination of Metallothionein in prostate tumor cell lines

GUMULEC, Jaromír, Michal MASAŘÍK, Šárka KUCHTÍČKOVÁ, Veronika KUDLÁČKOVÁ, Michal JURAJDA et. al.

Basic information

Original name

Determination of Metallothionein in prostate tumor cell lines

Name in Czech

Stanovení metalothioneinu v liniích karcinomu prostaty

Authors

GUMULEC, Jaromír, Michal MASAŘÍK, Šárka KUCHTÍČKOVÁ, Veronika KUDLÁČKOVÁ, Michal JURAJDA, Dušan PAVLÍK, Arne ROVNÝ, Roman HRABEC, Soňa KŘÍŽKOVÁ and René KIZEK

Edition

XIV. Setkání biochemiků a molekulárních biologů, 2010

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

30200 3.2 Clinical medicine

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Organization unit

Faculty of Medicine

ISBN

978-80-210-5164-5

Keywords (in Czech)

metalothionein;karcinom prostaty;nádorový marker;zinek

Keywords in English

metalothionein;prostate cancer;tumor marker;zinc;

Změněno: 29/11/2010 00:35, doc. MUDr. Jaromír Gumulec, Ph.D.

Abstract

ORIG CZ

V originále

Prostate carcinoma (PCa) is the most common tumor and second leading cause of death due to cancer of men. Proteins such as metallothionein (MT) or alpha-methyl-CoA-racemase (AMACR) are highly overexpressed in prostate carcinoma. Althrough the role of these proteins in tumour tissue remains still unclear, it fulfills the requirements of higher specificity, which AMACR achieves at almost 100%. We have analyzed both proteins from prostate cancer cell lines LNCaP, PC-3 and 22RVL, compared to healthy prostate cell line PNT1A and then from PCa patients' serum compared to healthy serum controls. We've used immunohistochemical analysis, immunoprecipitation with magnetic nanoparticles, PAGE and dot blots. In all of these methods, we've found significant increase of expression in these samples.

In Czech

Metalothionein (MT) a alpha-methyl-CoA-racemáza (AMACR) jsou výrazně exprimovány u karcinomu prostaty s velkou mírou specifity pro toto onemocnění. Tyto proteiny byly analyzovány u buněčných linií LNCaP, PC-3 a 22RVL a PNT1A a ve vzorcích sér pacientů. Bylo použito imunohistochemických metod, imonoprecipitace s magnetickými nanočásticemi, SDS-PAGE a dot bloty.

Links

GP301/09/P436, research and development project

Name: Analýza metalothioneinu u karcinomu prostaty na úrovni DNA, RNA a proteinu.

Investor: Czech Science Foundation

NS10200, research and development project

Name: Nádorové markery, jejich stanovení a korelace s karcinomem prostaty

Investor: Ministry of Health of the CR

Citovat

GUMULEC, Jaromír, Michal MASAŘÍK, Šárka KUCHTÍČKOVÁ, Veronika KUDLÁČKOVÁ, Michal JURAJDA, Dušan PAVLÍK, Arne ROVNÝ, Roman HRABEC, Soňa KŘÍŽKOVÁ and René KIZEK. Determination of Metallothionein in prostate tumor cell lines. In XIV. Setkání biochemiků a molekulárních biologů. 2010. ISBN 978-80-210-5164-5.

@proceedings{893719,
   author = {Gumulec, Jaromír and Masařík, Michal and Kuchtíčková, Šárka and Kudláčková, Veronika and Jurajda, Michal and Pavlík, Dušan and Rovný, Arne and Hrabec, Roman and Křížková, Soňa and Kizek, René},
   booktitle = {XIV. Setkání biochemiků a molekulárních biologů},
   keywords = {metalothionein;prostate cancer;tumor marker;zinc;},
   language = {eng},
   isbn = {978-80-210-5164-5},
   title = {Determination of Metallothionein in prostate tumor cell lines},
   url = {http://orion.chemi.muni.cz/setkani/Setkani14/index.htm},
   year = {2010}
}

TY  - CONF
ID  - 893719
AU  - Gumulec, Jaromír - Masařík, Michal - Kuchtíčková, Šárka - Kudláčková, Veronika - Jurajda, Michal - Pavlík, Dušan - Rovný, Arne - Hrabec, Roman - Křížková, Soňa - Kizek, René
PY  - 2010
TI  - Determination of Metallothionein in prostate tumor cell lines
SN  - 9788021051645
KW  - metalothionein;prostate cancer;tumor marker;zinc;
UR  - http://orion.chemi.muni.cz/setkani/Setkani14/index.htm
N2  - Prostate carcinoma (PCa) is the most common tumor and second leading cause of death due to cancer of men. Proteins such as metallothionein (MT) or alpha-methyl-CoA-racemase (AMACR) are highly overexpressed in prostate carcinoma. Althrough the role of these proteins in tumour tissue remains still unclear, it fulfills the requirements of higher specificity, which AMACR achieves at almost 100%. We have analyzed both proteins from prostate cancer cell lines LNCaP, PC-3 and 22RVL, compared to healthy prostate cell line PNT1A and then from PCa patients' serum compared to healthy serum controls. We've used immunohistochemical analysis, immunoprecipitation with magnetic nanoparticles, PAGE and dot blots. In all of these methods, we've found significant increase of expression in these samples.
ER  -

GUMULEC, Jaromír, Michal MASAŘÍK, Šárka KUCHTÍČKOVÁ, Veronika KUDLÁČKOVÁ, Michal JURAJDA, Dušan PAVLÍK, Arne ROVNÝ, Roman HRABEC, Soňa KŘÍŽKOVÁ and René KIZEK. Determination of Metallothionein in prostate tumor cell lines. In \textit{XIV. Setkání biochemiků a molekulárních biologů}. 2010. ISBN~978-80-210-5164-5.

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