Substrate-assisted laser desorption inductively-coupled plasma mass spectrometry for determination of biological microsamples

FOLTYNOVÁ, Pavla, Ondřej PEŠ, Tomáš VACULOVIČ, Jarmila NAVRÁTILOVÁ, Jan ŠMARDA, Viktor KANICKÝ and Jan PREISLER. Substrate-assisted laser desorption inductively-coupled plasma mass spectrometry for determination of biological microsamples. In Sborník 14. česko – slovenské spektroskopické konference. 2010. ISBN 978-80-7395-282-2.

Basic information

• Original name: Substrate-assisted laser desorption inductively-coupled plasma mass spectrometry for determination of biological microsamples
• Name in Czech: Analýza biologických vzorků pomocí SALD ICP MS
• Authors: FOLTYNOVÁ, Pavla (203 Czech Republic, belonging to the institution), Ondřej PEŠ (203 Czech Republic, belonging to the institution), Tomáš VACULOVIČ (203 Czech Republic, belonging to the institution), Jarmila NAVRÁTILOVÁ (203 Czech Republic, belonging to the institution), Jan ŠMARDA (203 Czech Republic, belonging to the institution), Viktor KANICKÝ (203 Czech Republic, belonging to the institution) and Jan PREISLER (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Sborník 14. česko – slovenské spektroskopické konference, 2010.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• RIV identification code: RIV/00216224:14310/10:00044535
• Organization unit: Faculty of Science
• ISBN: 978-80-7395-282-2
• Keywords (in Czech): SALD ICP MS; biologické vzork;y minimální množství vzork;ů kapilární elektroforéza; disulfiram; měď
• Keywords in English: SALD ICP MS; biological samples; minute amount of sample; capillary electrophoresis; disulfiram; copper

Abstract

A new method for determination of trace elements in submicroliter sample volumes using substrate-assisted laser desorption inductively-coupled plasma mass spectrometry (SALD ICP MS ) is presented. In contrast to conventional laser ablation (LA) ICP MS, SALD ICP MS allows quantitative analysis of metals in liquid samples because of complete sample desorption, which can be achieved using a few pulses with spot-to-spot reproducibility below 6 %. Detailed characterization, such as laser power density, raster and point desorption optimization etc. will be discussed. The performance of off-line coupling capillary electrophoresis and SALD ICP was demonstrated on speciation of 1 pg of Cr(III) and Cr (VI). SALD ICP MS can be used for the determination of trace elements in minute amounts of biological samples at physiological level as demonstrated on the determination of copper in myeloid leukemia cells to investigate the role of copper ions in cytotoxicity regulation with disulfiram .

Abstract (in Czech)

viz ang. text

Links

• GA203/09/1025, research and development project: Name: Multidetekční platforma pro mikrokolonové separace proteinů a peptidů

Investor: Czech Science Foundation, Multidetection platform for microcolumn separation of proteins and peptides

• MSM0021622411, plan (intention): Name: Studium a aplikace plazmochemických reakcí v neizotermickém nízkoteplotním plazmatu a jeho interakcí s povrchem pevných látek

Investor: Ministry of Education, Youth and Sports of the CR, Study and application of plasma chemical reactions in non-isothermic low temperature plasma and its interaction with solid surface

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations