Experimental Methods for Simultaneous Measurement of Action
Potentials and Electrograms in Isolated Heart

J 2010

Experimental Methods for Simultaneous Measurement of Action Potentials and Electrograms in Isolated Heart

KOLÁŘOVÁ, Jana, Kateřina FIALOVÁ, Oto JANOUŠEK, Marie NOVÁKOVÁ, Ivo PROVAZNÍK et. al.

Basic information

Original name

Experimental Methods for Simultaneous Measurement of Action Potentials and Electrograms in Isolated Heart

Authors

KOLÁŘOVÁ, Jana (203 Czech Republic, guarantor), Kateřina FIALOVÁ (203 Czech Republic), Oto JANOUŠEK (203 Czech Republic), Marie NOVÁKOVÁ (203 Czech Republic) and Ivo PROVAZNÍK (203 Czech Republic)

Edition

Physiological Research, 2010, 0862-8408

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.646

RIV identification code

RIV/00216224:14110/10:00044615

Organization unit

Faculty of Medicine

UT WoS

000279049100011

Keywords (in Czech)

Napěťově citlivá barviva; Pohybový artefakt; Ischemie; Vápník

Keywords in English

Voltage-Sensitive Dye; Motion Artifact; Ischemia; Calcium

Abstract

V originále

Monophasic action potential (MAP) can be recorded from the heart surface by optical method based on fluorescence measurement. The motion of isolated heart during experiment caused additional noise in recorded signal. The motion artifact can be eliminated by ratiometric fluorescence emission measurements. This study is based on experiments in which optical MAP measurement is done by single-wavelength and dual-wavelength measurement of fluorescence emission. Both recording setups are presented and their advantages and disadvantages are discussed. MAPs recorded by both methods from isolated rabbit hearts perfused according to Langendorff are presented. Simultaneous electrograms (EG) and MAPs recording are analyzed and measurement of velocity of impulse conduction through heart tissue is presented.

Links

GA102/07/1473, research and development project

Name: Optické metody registrace elektrických potenciálů a koncentrace vápníku v srdci s laserovou stabilizací

Investor: Czech Science Foundation

MSM0021622402, plan (intention)

Name: Časná diagnostika a léčba kardiovaskulárních chorob

Investor: Ministry of Education, Youth and Sports of the CR, Early diagnostics and treatment of cardiovascular diseases

Citovat

KOLÁŘOVÁ, Jana, Kateřina FIALOVÁ, Oto JANOUŠEK, Marie NOVÁKOVÁ and Ivo PROVAZNÍK. Experimental Methods for Simultaneous Measurement of Action Potentials and Electrograms in Isolated Heart. Physiological Research. 2010, vol. 59, Suppl 1, p. S71-S80, 10 pp. ISSN 0862-8408.

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   author = {Kolářová, Jana and Fialová, Kateřina and Janoušek, Oto and Nováková, Marie and Provazník, Ivo},
   article_number = {Suppl 1},
   keywords = {Voltage-Sensitive Dye; Motion Artifact; Ischemia; Calcium},
   language = {eng},
   issn = {0862-8408},
   journal = {Physiological Research},
   title = {Experimental Methods for Simultaneous Measurement of Action Potentials and Electrograms in Isolated Heart},
   url = {http://apps.isiknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=2&SID=P2OFFFD6GA56C3HOpNO&page=1&doc=1},
   volume = {vol. 59},
   year = {2010}
}

TY  - JOUR
ID  - 895673
AU  - Kolářová, Jana - Fialová, Kateřina - Janoušek, Oto - Nováková, Marie - Provazník, Ivo
PY  - 2010
TI  - Experimental Methods for Simultaneous Measurement of Action Potentials and Electrograms in Isolated Heart
JF  - Physiological Research
VL  - vol. 59
IS  - Suppl 1
SP  - S71-S80
EP  - S71-S80
SN  - 08628408
KW  - Voltage-Sensitive Dye
KW  - Motion Artifact
KW  - Ischemia
KW  - Calcium
UR  - http://apps.isiknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=2&SID=P2OFFFD6GA56C3HOpNO&page=1&doc=1
N2  - Monophasic action potential (MAP) can be recorded from the heart surface by optical method based on fluorescence measurement. The motion of isolated heart during experiment caused additional noise in recorded signal. The motion artifact can be eliminated by ratiometric fluorescence emission measurements. This study is based on experiments in which optical MAP measurement is done by single-wavelength and dual-wavelength measurement of fluorescence emission. Both recording setups are presented and their advantages and disadvantages are discussed. MAPs recorded by both methods from isolated rabbit hearts perfused according to Langendorff are presented. Simultaneous electrograms (EG) and MAPs recording are analyzed and measurement of velocity of impulse conduction through heart tissue is presented.
ER  -

KOLÁŘOVÁ, Jana, Kateřina FIALOVÁ, Oto JANOUŠEK, Marie NOVÁKOVÁ and Ivo PROVAZNÍK. Experimental Methods for Simultaneous Measurement of Action Potentials and Electrograms in Isolated Heart. \textit{Physiological Research}. 2010, vol. 59, Suppl 1, p.~S71-S80, 10 pp. ISSN~0862-8408.

Detailed Information on Publication Record