Experimental Methods for Simultaneous Measurement of Action Potentials and Electrograms in Isolated Heart

KOLÁŘOVÁ, Jana, Kateřina FIALOVÁ, Oto JANOUŠEK, Marie NOVÁKOVÁ and Ivo PROVAZNÍK. Experimental Methods for Simultaneous Measurement of Action Potentials and Electrograms in Isolated Heart. Physiological Research. 2010, vol. 59, Suppl 1, p. S71-S80, 10 pp. ISSN 0862-8408.

Basic information

• Original name: Experimental Methods for Simultaneous Measurement of Action Potentials and Electrograms in Isolated Heart
• Authors: KOLÁŘOVÁ, Jana (203 Czech Republic, guarantor), Kateřina FIALOVÁ (203 Czech Republic), Oto JANOUŠEK (203 Czech Republic), Marie NOVÁKOVÁ (203 Czech Republic) and Ivo PROVAZNÍK (203 Czech Republic).
• Edition: Physiological Research, 2010, 0862-8408.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 30105 Physiology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 1.646
• RIV identification code: RIV/00216224:14110/10:00044615
• Organization unit: Faculty of Medicine
• UT WoS: 000279049100011
• Keywords (in Czech): Napěťově citlivá barviva; Pohybový artefakt; Ischemie; Vápník
• Keywords in English: Voltage-Sensitive Dye; Motion Artifact; Ischemia; Calcium
• Tags: International impact, Reviewed

Abstract

Monophasic action potential (MAP) can be recorded from the heart surface by optical method based on fluorescence measurement. The motion of isolated heart during experiment caused additional noise in recorded signal. The motion artifact can be eliminated by ratiometric fluorescence emission measurements. This study is based on experiments in which optical MAP measurement is done by single-wavelength and dual-wavelength measurement of fluorescence emission. Both recording setups are presented and their advantages and disadvantages are discussed. MAPs recorded by both methods from isolated rabbit hearts perfused according to Langendorff are presented. Simultaneous electrograms (EG) and MAPs recording are analyzed and measurement of velocity of impulse conduction through heart tissue is presented.

Links

• GA102/07/1473, research and development project: Name: Optické metody registrace elektrických potenciálů a koncentrace vápníku v srdci s laserovou stabilizací

Investor: Czech Science Foundation

• MSM0021622402, plan (intention): Name: Časná diagnostika a léčba kardiovaskulárních chorob

Investor: Ministry of Education, Youth and Sports of the CR, Early diagnostics and treatment of cardiovascular diseases