Detailed Information on Publication Record
2010
The role of chromatin condensation during granulopoiesis in the regulation of gene cluster expression
STEJSKAL, Stanislav, Irena KRONTORÁD KOUTNÁ, Pavel MATULA, Zdeněk RUČKA, Ondřej DANĚK et. al.Basic information
Original name
The role of chromatin condensation during granulopoiesis in the regulation of gene cluster expression
Name in Czech
Role chromatinové kondenzace v regulaci genových lokusů během granulopoézy
Authors
STEJSKAL, Stanislav (203 Czech Republic, belonging to the institution), Irena KRONTORÁD KOUTNÁ (203 Czech Republic, guarantor, belonging to the institution), Pavel MATULA (203 Czech Republic, belonging to the institution), Zdeněk RUČKA (203 Czech Republic, belonging to the institution), Ondřej DANĚK (203 Czech Republic, belonging to the institution), Martin MAŠKA (203 Czech Republic, belonging to the institution) and Michal KOZUBEK (203 Czech Republic, belonging to the institution)
Edition
Epigenetics, USA, Landes Bioscience, 2010, 1559-2294
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 4.622
RIV identification code
RIV/00216224:14330/10:00044622
Organization unit
Faculty of Informatics
UT WoS
000284263500012
Keywords (in Czech)
Granulocyty; Imunohistochemie; RNA polymerasa II; Analýza obrazu; buněčná diferenciace
Keywords in English
Granulocytes; Immunohistochemistry; RNA polymerase II; Image analysis; Cell differentiation
Tags
Tags
International impact, Reviewed
Změněno: 13/4/2012 00:35, doc. RNDr. Pavel Matula, Ph.D.
Abstract
V originále
Changes in nuclear architecture play an important role in the regulation of gene expression. The importance of epigenetic changes is observed during granulopoiesis, when changes in the nuclear architecture are considered a major factor that influences the downregulation of genes. We aimed to assess the influence of chromatin condensation on the regulation of gene expression during granulopoiesis. Based on a previously published microarray analysis, we chose loci with different levels of transcriptional activity during granulopoiesis. Fluorescent in situ hybridisation (FISH) and immunofluorescent labelling of RNA polymerase II were used to determine the relationship between the transcriptional activity of gene clusters and their localisation within areas with different levels of chromatin condensation. Although active loci were positioned outside of areas of condensed chromatin, downregulation of genes during granulopoiesis was not accompanied by a shift of the downregulated loci to condensed areas. Only the beta-globin cluster was subjected to chromatin condensation and localised to condensed areas. Our results indicate that granulopoiesis is accompanied by a non-random, tissue-specific pattern of chromatin condensation. Furthermore, we observed that the decrease in the quantity of RNA polymerase II correlates with the differentiation process and likely acts in synergy with chromatin condensation to downregulate total gene expression.
Links
| MSM0021622419, plan (intention) |
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| MSM0021622430, plan (intention) |
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| 2B06052, research and development project |
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