Inhibition of gap-junctional intercellular communication and activation of MAPKs by cyanobacterial extracts

ADAMOVSKÝ, Ondřej, Luděk BLÁHA, Pavel BABICA, Klára HILSCHEROVÁ and Brad UPHAM. Inhibition of gap-junctional intercellular communication and activation of MAPKs by cyanobacterial extracts. In SETAC, Seville 2010. 2010.

Basic information

• Original name: Inhibition of gap-junctional intercellular communication and activation of MAPKs by cyanobacterial extracts
• Name in Czech: Inhibice mezibuňečné komunikace a inhibice MAP kináz sinicovými extrakty
• Authors: ADAMOVSKÝ, Ondřej (203 Czech Republic, guarantor), Luděk BLÁHA (203 Czech Republic), Pavel BABICA (203 Czech Republic), Klára HILSCHEROVÁ (203 Czech Republic) and Brad UPHAM (840 United States of America).
• Edition: SETAC, Seville 2010, 2010.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 30304 Public and environmental health
• Country of publisher: Spain
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/10:00048813
• Organization unit: Faculty of Science
• Keywords (in Czech): sinocové toxiny promoce karcinogeneze
• Keywords in English: cyanobacterial toxins tumor promotion

Abstract

Cyanobacteria produce various biologically active compounds which might represent a cancer risk. We investigated effects of various cyanobacterial extracts and pure cyanotoxin microcystin-LR on inhibition of gap-junctional intercellular communication (GJIC) along with activations of mitogen-activated protein kinases (MAPKs) in normal rat liver stem-like WB-F344 cell line. Pure microcystin did not inhibit GJIC or activate MAPKs, but complex extracts of water blooms significantly inhibited GJIC and activated MAPK, independent from the content of microcystin. The most pronounced effects were systematically observed in extracts of the cultures of Aphanizomenon flos-aquae as well as in complex water bloom extract dominated by the same species, which does not produce microcystin. Microcystis sp. culture or water bloom had significant but less pronounced effects on both GJIC inhibition and MAPK activation. These are the first results indicating the involvement of cyanobacteria toxins in the regulation of GJIC and MAPK, and also that tumor promoting metabolites other than microcystins are present.

Abstract (in Czech)

Cyanobacteria produce various biologically active compounds which might represent a cancer risk. We investigated effects of various cyanobacterial extracts and pure cyanotoxin microcystin-LR on inhibition of gap-junctional intercellular communication (GJIC) along with activations of mitogen-activated protein kinases (MAPKs) in normal rat liver stem-like WB-F344 cell line. Pure microcystin did not inhibit GJIC or activate MAPKs, but complex extracts of water blooms significantly inhibited GJIC and activated MAPK, independent from the content of microcystin. The most pronounced effects were systematically observed in extracts of the cultures of Aphanizomenon flos-aquae as well as in complex water bloom extract dominated by the same species, which does not produce microcystin. Microcystis sp. culture or water bloom had significant but less pronounced effects on both GJIC inhibition and MAPK activation. These are the first results indicating the involvement of cyanobacteria toxins in the regulation of GJIC and MAPK, and also that tumor promoting metabolites other than microcystins are present.

Links

• GA524/08/0496, research and development project: Name: Mechanismy nádorové promoce metabolitů toxických sinic

Investor: Czech Science Foundation, Tumor promotional mechanisms of cyanobacterial metabolites