HRNČÍŘOVÁ, Kristýna, Martina LENGEROVÁ, Iva KOCMANOVÁ, Zdeněk RÁČIL, Pavlína VOLFOVÁ, Dita PALOUŠOVÁ, Mojmír MOULIS, Barbora WEINBERGEROVÁ, Jana WINTEROVÁ, Martina TOŠKOVÁ, Šárka POSPÍŠILOVÁ and Jiří MAYER. Rapid detection and identification of mucormycetes from culture and tissue samples by use of high-resolution melt analysis. Journal of Clinical Microbiology. Washington: American Society for Microbiology, 2010, vol. 48, No 9, p. 3392-3394. ISSN 0095-1137. Available from: https://dx.doi.org/10.1128/JCM.01109-10.
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Basic information
Original name Rapid detection and identification of mucormycetes from culture and tissue samples by use of high-resolution melt analysis
Name in Czech Rychlá detekce a identifikace zygomycet z kultur a vzorků tkáně pomocí High Resolution Melt analýzy
Authors HRNČÍŘOVÁ, Kristýna (203 Czech Republic, guarantor, belonging to the institution), Martina LENGEROVÁ (203 Czech Republic, belonging to the institution), Iva KOCMANOVÁ (203 Czech Republic, belonging to the institution), Zdeněk RÁČIL (203 Czech Republic, belonging to the institution), Pavlína VOLFOVÁ (203 Czech Republic, belonging to the institution), Dita PALOUŠOVÁ (203 Czech Republic, belonging to the institution), Mojmír MOULIS (203 Czech Republic, belonging to the institution), Barbora WEINBERGEROVÁ (203 Czech Republic), Jana WINTEROVÁ (703 Slovakia, belonging to the institution), Martina TOŠKOVÁ (703 Slovakia, belonging to the institution), Šárka POSPÍŠILOVÁ (203 Czech Republic, belonging to the institution) and Jiří MAYER (203 Czech Republic, belonging to the institution).
Edition Journal of Clinical Microbiology, Washington, American Society for Microbiology, 2010, 0095-1137.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30200 3.2 Clinical medicine
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 4.220
RIV identification code RIV/00216224:14110/10:00051446
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1128/JCM.01109-10
UT WoS 000281480400063
Keywords in English Mucormycetes; rapid detection; simple detection; real-time PCR
Tags International impact
Changed by Changed by: Mgr. Michal Petr, učo 65024. Changed: 20/4/2012 14:59.
Abstract
We present a method for rapid and simple detection of clinically relevant mucormycetes of the Mucorales order in cultures and clinical samples. This seminested real-time PCR uses mucormycete-specific primers and is followed by species identification using high-resolution melt (HRM) analysis. The method is highly suitable for routine clinical diagnostics.
Abstract (in Czech)
Představujeme metodu pro rychlou a jednoduchou detekci klinicky důležitých zygomycet řádu Mucorales z kultur a klinických vzorků. Tato seminested real-time PCR využívá primery specifické pro zygomycety a je následována rozlišením jednotlivých druhů pomocí High Resolution Melt (HRM) analýzy. Tato metoda je velmi vhodná pro rutinní laboratorní diagnostiku.
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