a 2010

Determination of the surface pathology of human sperm by atomic force microscopy

CRHA, Igor, Jan PŘIBYL, Petr SKLÁDAL, Jana ŽÁKOVÁ, Pavel VENTRUBA et. al.

Basic information

Original name

Determination of the surface pathology of human sperm by atomic force microscopy

Name in Czech

Zobrazení patologií povrchu spermie metodou mikroskopie atomových sil0268-1161

Authors

CRHA, Igor (203 Czech Republic, guarantor, belonging to the institution), Jan PŘIBYL (203 Czech Republic, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution), Jana ŽÁKOVÁ (203 Czech Republic, belonging to the institution), Pavel VENTRUBA (203 Czech Republic, belonging to the institution) and Michal POHANKA (203 Czech Republic, belonging to the institution)

Edition

26th Annual Meeting of ESHRE, 2010

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

30211 Orthopaedics

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 4.357

RIV identification code

RIV/00216224:14110/10:00058891

Organization unit

Faculty of Medicine

ISSN

UT WoS

000279875400293

Keywords (in Czech)

spermie; AFM; neplodnost

Keywords in English

sperm; AFM; infertility

Tags

International impact
Změněno: 4/10/2012 12:19, Mgr. Michal Petr

Abstract

V originále

Atomic force microscopy (AFM) employs a sharp probe for profiling surfaces with unique resolution. This technique has developed into an invaluable multidisciplinary tool for advanced characterization of different samples. In its basic application, AFM provides high resolution imaging of surface structures at scales ranging from a few nanometers to hundreds micrometers. Oxidative stress is considered one of the basic etiological factors of the male’s fertility disorders. Excessive production of oxygen radicals results in damage to the sperm’s cell membrane and to the DNA. The collected AFM images clearly highlight many details of normal spermatozoa and spermatozoa damaged by hydrogen peroxide. This technique could be an important tool in the research of oxidative stress and understanding its effect on male infertility.

Links

MSM0021622413, plan (intention)
Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment
NS9661, research and development project
Name: Vliv metabolismu homocysteinu na poruchy plodnosti.
Investor: Ministry of Health of the CR