Determination of the surface pathology of human sperm by atomic force microscopy

CRHA, Igor, Jan PŘIBYL, Petr SKLÁDAL, Jana ŽÁKOVÁ, Pavel VENTRUBA and Michal POHANKA. Determination of the surface pathology of human sperm by atomic force microscopy. In 26th Annual Meeting of ESHRE. 2010. ISSN 0268-1161.

Basic information

• Original name: Determination of the surface pathology of human sperm by atomic force microscopy
• Name in Czech: Zobrazení patologií povrchu spermie metodou mikroskopie atomových sil0268-1161
• Authors: CRHA, Igor (203 Czech Republic, guarantor, belonging to the institution), Jan PŘIBYL (203 Czech Republic, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution), Jana ŽÁKOVÁ (203 Czech Republic, belonging to the institution), Pavel VENTRUBA (203 Czech Republic, belonging to the institution) and Michal POHANKA (203 Czech Republic, belonging to the institution).
• Edition: 26th Annual Meeting of ESHRE, 2010.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 30211 Orthopaedics
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 4.357
• RIV identification code: RIV/00216224:14110/10:00058891
• Organization unit: Faculty of Medicine
• ISSN: 0268-1161
• UT WoS: 000279875400293
• Keywords (in Czech): spermie; AFM; neplodnost
• Keywords in English: sperm; AFM; infertility
• Tags: International impact

Abstract

Atomic force microscopy (AFM) employs a sharp probe for profiling surfaces with unique resolution. This technique has developed into an invaluable multidisciplinary tool for advanced characterization of different samples. In its basic application, AFM provides high resolution imaging of surface structures at scales ranging from a few nanometers to hundreds micrometers. Oxidative stress is considered one of the basic etiological factors of the male’s fertility disorders. Excessive production of oxygen radicals results in damage to the sperm’s cell membrane and to the DNA. The collected AFM images clearly highlight many details of normal spermatozoa and spermatozoa damaged by hydrogen peroxide. This technique could be an important tool in the research of oxidative stress and understanding its effect on male infertility.

Links

• MSM0021622413, plan (intention): Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment

• NS9661, research and development project: Name: Vliv metabolismu homocysteinu na poruchy plodnosti.

Investor: Ministry of Health of the CR