J 2010

Ethanol-fixed material used for both classical and molecular identification purposes: Eudiplozoon nipponicum (Monogenea: Diplozoidae) as a case parasite species

KOŠKOVÁ, Eva, Iveta MATĚJUSOVÁ, Kristína CIVÁŇOVÁ and Božena KOUBKOVÁ

Basic information

Original name

Ethanol-fixed material used for both classical and molecular identification purposes: Eudiplozoon nipponicum (Monogenea: Diplozoidae) as a case parasite species

Authors

KOŠKOVÁ, Eva (203 Czech Republic), Iveta MATĚJUSOVÁ (203 Czech Republic), Kristína CIVÁŇOVÁ (703 Slovakia) and Božena KOUBKOVÁ (203 Czech Republic, guarantor)

Edition

Parasitology Research, Heidelberg, Springer-Verlag, 2010, 0932-0113

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 1.812

RIV identification code

RIV/00216224:14310/10:00044906

Organization unit

Faculty of Science

UT WoS

000281673800018

Keywords (in Czech)

sodium dodecyl sulphat; ethanol fixed material; Diplozoidae

Keywords in English

sodium dodecyl sulphat;ethanol fixed material; Diplozoidae

Tags

International impact
Změněno: 30/9/2010 13:14, RNDr. Božena Koubková, Ph.D.

Abstract

V originále

This study is focused on the feasibility of two treatments of alcohol-fixed monogenean parasites which are intended to be use for the combined morphological and molecular characterizations. The monogenean parasite, Eudiplozoon nipponicum, was selected as a model parasite species; however it is expected that these techniques will be suitable for other monogeneans and other parasitic families. The haptor of diplozoid parasites is equipped with sclerotized attachment clamps and central hooks which are utilized for morphological identification. As parasite tissue become very tough and rigid when preserved in ethanol, using these structures for species identification without additional treatment is difficult. We investigated two different techniques to digest the surrounding tissues, the first was treatment with 10% sodium dodecyl sulphate (SDS) and the second treatment was proteinase K.

Links

GA524/07/1610, research and development project
Name: Plasticita fenotypu monogeneí: kombinace molekulárního a morfologického přístupu
Investor: Czech Science Foundation, Phenotypic plasticity of monogenean parasites: a combined molecular and morphological approach
GD526/09/H025, research and development project
Name: Evolučně-ekologická analýza společenstev a populací
Investor: Czech Science Foundation, Evolutionary ecological analysis of communities and populations
LC522, research and development project
Name: ICHTYOPARAZITOLOGIE - centrum základního výzkumu
Investor: Ministry of Education, Youth and Sports of the CR, Ichtyoparazitology - the center of basic research
MSM0021622416, plan (intention)
Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase
Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time