Candidate markers for powdery mildew resistance genes from wild barley PI284752

ŘEPKOVÁ, Jana and Antonín DREISEITL. Candidate markers for powdery mildew resistance genes from wild barley PI284752. Euphytica. Nizozemí: Springer, 2010, vol. 175, No 3, p. 283-292. ISSN 0014-2336.

Basic information

• Original name: Candidate markers for powdery mildew resistance genes from wild barley PI284752
• Name in Czech: Kandidátní markery genů odolnosti k padlí travnímu planého ječmene PI284752
• Authors: ŘEPKOVÁ, Jana (203 Czech Republic, guarantor, belonging to the institution) and Antonín DREISEITL (203 Czech Republic).
• Edition: Euphytica, Nizozemí, Springer, 2010, 0014-2336.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: Genetics and molecular biology
• Country of publisher: Netherlands
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 1.597
• RIV identification code: RIV/00216224:14310/10:00044918
• Organization unit: Faculty of Science
• UT WoS: 000281380400001
• Keywords (in Czech): Blumeria graminis f.sp. hordei; genetické mapování; Hordeum vulgare ssp. spontaneum; resistance gene homolog; markery asistovaná selekce
• Keywords in English: Blumeria graminis f.sp. hordei; genetic mapping; Hordeum vulgare ssp. spontaneum; resistance gene homolog; marker-assisted selection

Abstract

PI284752, an accession of wild barley (Hordeum vulgare ssp. spontaneum) resistant to powdery mildew caused by Blumeria graminis f.sp. hordei, was studied with the aim of identifying genes involved in powdery mildew resistance. An F-2 population (456 plants) was established from a cross between the winter barley variety 'Tiffany' and PI284752. This cross demonstrated a two-locus model of resistance. Linkage analysis using polymorphic DNA markers was carried out on 180 plants. The RGH1a gene sequence from the Mla locus was used as a source for developing the RGH1aE2I2 marker. One resistance gene was found to be tightly linked with RGH1aE2I2 on chromosome 1HS and it was found to be located 2 cM from GBMS062. In plants with RT1 to RT2-3, the resistance was conferred exclusively by the second R gene which is linked with Bmac0134 and GBMS247 on chromosome 2HS. The aforementioned markers may be valuable candidates for marker-assisted selection of resistant genotypes.

Abstract (in Czech)

PI284752, planý ječmen (Hordeum vulgare ssp. spontaneum) odolný k padlí travnímu způsobenému houbou Blumeria graminis f.sp. hordei, by analyzován s cílem identifikace genů odolnosti a nalezení kandidátních markerů těchto genů vhodných pro markery asistovanou selekci (MAS). Populace F2 (456 rostlin) byla získána křížením odrůdy kulturního ječmene 'Tiffany' a PI284752. Odolnost byla determinována dvěma lokusy a prostřednictvím genetického mapování byly tyto lokusy lokalizovány na chromozomy 1H a 2M. Byly identifikovány markery RGH1aE2I2 a GBMS062 na chromozomu 1H a markery Bmac0134 a GBMS247 na chromozomu 2H vhodné pro efektivní MAS.

Links

• GA522/06/0608, research and development project: Name: Vývoj DNA markerů a konstrukce genetické mapy k lokalizaci nových genů odolnosti ječmene ozimého k padlí travnímu

Investor: Czech Science Foundation

• MSM 143100008, plan (intention): Name: Genomy a jejich funkce

Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions