HPLC determination of caffeine and paraxanthine in saliva as a method for CYP1A2 metabolic activity assessment in humans

JUŘICA, Jan, Ondřej ZENDULKA and Josef TOMANDL. HPLC determination of caffeine and paraxanthine in saliva as a method for CYP1A2 metabolic activity assessment in humans. In 28th. International symposium on chromatography. 2010.

Basic information

• Original name: HPLC determination of caffeine and paraxanthine in saliva as a method for CYP1A2 metabolic activity assessment in humans
• Name in Czech: Stanovení kofeinu a paraxantinu ve slinách jako metoda pro stanovení metabolické aktivity CYP1A2 u lidí
• Authors: JUŘICA, Jan, Ondřej ZENDULKA and Josef TOMANDL.
• Edition: 28th. International symposium on chromatography, 2010.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 30104 Pharmacology and pharmacy
• Country of publisher: Spain
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Medicine
• Keywords (in Czech): CYP1A2; metabolická aktivita, kofein
• Keywords in English: CYP1A2; metabolic activity, caffein
• Tags: caffein, CYP1A2, paraxanthine
• Tags: International impact

Abstract

The mehod for simple and reliable determination of caffein and paraxanthine in human saliva was developed. After simple, liquid-liquid extraction into organic solvents, sample were separated on Kinetex PFP column and detected at 268 nm. Mobile phase (0.05% acetic acid: methanol, 75:25) was eluted isocratically at flow rate of 0.55 ml/min. All analytes wre eluted within 13 min.

Abstract (in Czech)

Byla vyvinuta rychlá a aspolehlivá metoda pro stanovení kofeinu a paraxanthinu v lidských slinách jako metoda pro sledvání metabolické aktivity 1A2isoformy cytochromu P450. Po jednoduché extrakci do organického rozpouštědla byly vzorky děleny na koloně Kinetex PFP a detekovány při 268 nm. Průtok mobilní fáze (0.05% octová kyselina: methanol, 75:25)byl konstantní (0,55ml/min. Všechny analyty byly separována během 13 min.

Links

• LC06023, research and development project: Name: Integrované bioanalytické technologie pro mikroanalýzy a diagnostiku s využitím LIF a hmotnostní spektrometrie

Investor: Ministry of Education, Youth and Sports of the CR

• MSM0021622404, plan (intention): Name: Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS

Investor: Ministry of Education, Youth and Sports of the CR, The internal organisation and neurobiological mechanisms of functional CNS systems under normal and pathological conditions.