Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering

PROKOP, Zbyněk, Yukari SATO, Jan BREZOVSKÝ, Tomáš MOZGA, Radka CHALOUPKOVÁ, Táňa KOUDELÁKOVÁ, Petr JEŘÁBEK, Veronika ŠTĚPÁNKOVÁ, Rio NATSUME, J.G.E. LEEWEN, D,B. JANSSEN, J. FLORIAN, Yuji NAGATA, T. SENDA and Jiří DAMBORSKÝ. Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering. Angewandte Chemie International Edition. Wiley-VCH Verlag GmbH & Co. KGaA, Weinhe, 2010, vol. 49, No 35, p. 6111-6115. ISSN 1433-7851.

Basic information

• Original name: Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering
• Authors: PROKOP, Zbyněk (203 Czech Republic, belonging to the institution), Yukari SATO (392 Japan), Jan BREZOVSKÝ (203 Czech Republic, belonging to the institution), Tomáš MOZGA (203 Czech Republic), Radka CHALOUPKOVÁ (203 Czech Republic, belonging to the institution), Táňa KOUDELÁKOVÁ (203 Czech Republic, belonging to the institution), Petr JEŘÁBEK (203 Czech Republic), Veronika ŠTĚPÁNKOVÁ (203 Czech Republic, belonging to the institution), Rio NATSUME (392 Japan), J.G.E. LEEWEN (528 Netherlands), D,B. JANSSEN (528 Netherlands), J. FLORIAN (203 Czech Republic), Yuji NAGATA (392 Japan), T. SENDA (392 Japan) and Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Angewandte Chemie International Edition, Wiley-VCH Verlag GmbH & Co. KGaA, Weinhe, 2010, 1433-7851.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Germany
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 12.730
• RIV identification code: RIV/00216224:14310/10:00040598
• Organization unit: Faculty of Science
• UT WoS: 000281316000011
• Keywords in English: Enantioselectivity; Haloalkane Dehalogenases; protein engineering

Abstract

Engineering of the surface loop in haloalkane dehalogenases affects their enantiodiscrimination behavior. The temperature dependence of the enantioselectivity (lnE versus 1/T) of -bromoalkanes by haloalkane dehalogenases is reversed (red data points) by deletion of the surface loop; the selectivity switches back when an additional single-point mutation is made. This behavior is not observed for -bromoesters.

Abstract (in Czech)

nženýrství povrchu vedení v halogenalkandehalogenáz ovlivňuje jejich enantiodiskriminační chování. Teplotní závislost enantioselektivity (LNE versus 1 / T)-bromoalkánů od halogenalkandehalogenáz je opačný od vypuštěné plochy smyčky, selektivita přepne zpět, když existují další jedno-bodové mutace. Toto chování není pozorováno u bromoesterů.

Links

• IAA401630901, research and development project: Name: Evoluce substrátové specifity u enzymů aktivních s xenobiotickými látkami

Investor: Academy of Sciences of the Czech Republic, Evolution of substrate specificity in enzymes acting on xenobiotic compounds

• LC06010, research and development project: Name: Centrum biokatalýzy a biotransformací

Investor: Ministry of Education, Youth and Sports of the CR, Center of Biocatalysis and Biotransformation

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)