Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering

PROKOP, Zbyněk, Yukari SATO, Jan BREZOVSKÝ, Tomáš MOZGA, Radka CHALOUPKOVÁ, Táňa KOUDELÁKOVÁ, Petr JEŘÁBEK, Veronika ŠTĚPÁNKOVÁ, Rio NATSUME, J.G.E. LEEWEN, D,B. JANSSEN, J. FLORIAN, Yuji NAGATA, T. SENDA and Jiří DAMBORSKÝ. Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering. Angewandte Chemie International Edition. Wiley-VCH Verlag GmbH & Co. KGaA, Weinhe, 2010, vol. 49, No 35, p. 6111-6115. ISSN 1433-7851.

Basic information

Original name

Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering

Authors

PROKOP, Zbyněk (203 Czech Republic, belonging to the institution), Yukari SATO (392 Japan), Jan BREZOVSKÝ (203 Czech Republic, belonging to the institution), Tomáš MOZGA (203 Czech Republic), Radka CHALOUPKOVÁ (203 Czech Republic, belonging to the institution), Táňa KOUDELÁKOVÁ (203 Czech Republic, belonging to the institution), Petr JEŘÁBEK (203 Czech Republic), Veronika ŠTĚPÁNKOVÁ (203 Czech Republic, belonging to the institution), Rio NATSUME (392 Japan), J.G.E. LEEWEN (528 Netherlands), D,B. JANSSEN (528 Netherlands), J. FLORIAN (203 Czech Republic), Yuji NAGATA (392 Japan), T. SENDA (392 Japan) and Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Angewandte Chemie International Edition, Wiley-VCH Verlag GmbH & Co. KGaA, Weinhe, 2010, 1433-7851

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 12.730

RIV identification code

RIV/00216224:14310/10:00040598

Organization unit

Faculty of Science

UT WoS

000281316000011

Keywords in English

Enantioselectivity; Haloalkane Dehalogenases; protein engineering

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Abstract

V originále

Engineering of the surface loop in haloalkane dehalogenases affects their enantiodiscrimination behavior. The temperature dependence of the enantioselectivity (lnE versus 1/T) of -bromoalkanes by haloalkane dehalogenases is reversed (red data points) by deletion of the surface loop; the selectivity switches back when an additional single-point mutation is made. This behavior is not observed for -bromoesters.

In Czech

nženýrství povrchu vedení v halogenalkandehalogenáz ovlivňuje jejich enantiodiskriminační chování. Teplotní závislost enantioselektivity (LNE versus 1 / T)-bromoalkánů od halogenalkandehalogenáz je opačný od vypuštěné plochy smyčky, selektivita přepne zpět, když existují další jedno-bodové mutace. Toto chování není pozorováno u bromoesterů.

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Links

IAA401630901, research and development project	Name: Evoluce substrátové specifity u enzymů aktivních s xenobiotickými látkami Investor: Academy of Sciences of the Czech Republic, Evolution of substrate specificity in enzymes acting on xenobiotic compounds
LC06010, research and development project	Name: Centrum biokatalýzy a biotransformací Investor: Ministry of Education, Youth and Sports of the CR, Center of Biocatalysis and Biotransformation
MSM0021622412, plan (intention)	Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL) Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)