Atomic resolution studies of haloalkane dehalogenases DhaA04, DhaA14 and DhaA15 with engineered access tunnels.

STSIAPANAVA, Alena, J. DOHNÁLEK, J. GAVIRA, Michal KUTÝ, Táňa KOUDELÁKOVÁ, Jiří DAMBORSKÝ and Ivana KUTÁ-SMATANOVÁ. Atomic resolution studies of haloalkane dehalogenases DhaA04, DhaA14 and DhaA15 with engineered access tunnels. ACTA CRYSTALLOGRAPHICA D. International Union of Crystallography, 2010, vol. 66, No 66, p. 962-969. ISSN 0907-4449.

Basic information

• Original name: Atomic resolution studies of haloalkane dehalogenases DhaA04, DhaA14 and DhaA15 with engineered access tunnels.
• Authors: STSIAPANAVA, Alena (112 Belarus), J. DOHNÁLEK (203 Czech Republic), J. GAVIRA (203 Czech Republic), Michal KUTÝ (203 Czech Republic), Táňa KOUDELÁKOVÁ (203 Czech Republic), Jiří DAMBORSKÝ (203 Czech Republic, guarantor) and Ivana KUTÁ-SMATANOVÁ (203 Czech Republic).
• Edition: ACTA CRYSTALLOGRAPHICA D, International Union of Crystallography, 2010, 0907-4449.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 6.326
• RIV identification code: RIV/00216224:14310/10:00045039
• Organization unit: Faculty of Science
• UT WoS: 000281635500002
• Keywords (in Czech): haloalkán dehalogenáza DhaA ; Rhodococcus rhodochrous NCIMB 13064; hydrolytická degradace; 1.2.3-trichloropropan (TCP)
• Keywords in English: haloalkane dehalogenase DhaA ; Rhodococcus rhodochrous NCIMB 13064; hydrolytic degradation; 1.2.3-trichloropropane (TCP)

Abstract

The haloalkane dehalogenase DhaA from Rhodococcus rhodochrous NCIMB 13064 is a bacterial enzyme that shows catalytic activity for the hydrolytic degradation of the highly toxic industrial pollutant 1,2,3-trichloropropane (TCP). Mutagenesis focused on the access tunnels of DhaA produced protein variants with significantly improved activity towards TCP. Three mutants of DhaA named DhaA04 (C176Y), DhaA14 (I135F) and DhaA15 (C176Y + I135F) were constructed in order to study the functional relevance of the tunnels connecting the buried active site of the protein with the surrounding solvent. All three protein variants were crystallized using the sitting-drop vapour-diffusion technique.

Links

• LC06010, research and development project: Name: Centrum biokatalýzy a biotransformací

Investor: Ministry of Education, Youth and Sports of the CR, Center of Biocatalysis and Biotransformation

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)

• MSM0021622413, plan (intention): Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment