| MOTÁČKOVÁ, Veronika, Jiří NOVÁČEK, Anna ZAWADZKA-KAZIMIERCZUK, Krzysztof KAZIMIERCZUK, Lukáš ŽÍDEK, Hana ŠANDEROVÁ, Libor KRÁSNÝ, Wiktor KOZMINSKI and Vladimír SKLENÁŘ. Strategy for complete NMR assignment of disordered proteins with highly repetitive sequences based on resolution-enhanced 5D experiments. Journal of Biomolecular NMR. Netherlands: Springer Netherlands, 2010, vol. 48, No 3, p. 169-177. ISSN 0925-2738. |
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@article{903212,
author = {Motáčková, Veronika and Nováček, Jiří and ZawadzkaandKazimierczuk, Anna and Kazimierczuk, Krzysztof and Žídek, Lukáš and Šanderová, Hana and Krásný, Libor and Kozminski, Wiktor and Sklenář, Vladimír},
article_location = {Netherlands},
article_number = {3},
keywords = {Unfolded proteins; Repetitive sequence; Multi-dimensional NMR; Non-uniform sampling; Assignment},
language = {eng},
issn = {0925-2738},
journal = {Journal of Biomolecular NMR},
title = {Strategy for complete NMR assignment of disordered proteins with highly repetitive sequences based on resolution-enhanced 5D experiments},
url = {http://www.springerlink.com/content/?k=doi%3a%2810.1007%2fs10858-010-9447-3%29},
volume = {48},
year = {2010}
}
TY - JOUR
ID - 903212
AU - Motáčková, Veronika - Nováček, Jiří - Zawadzka-Kazimierczuk, Anna - Kazimierczuk, Krzysztof - Žídek, Lukáš - Šanderová, Hana - Krásný, Libor - Kozminski, Wiktor - Sklenář, Vladimír
PY - 2010
TI - Strategy for complete NMR assignment of disordered proteins with highly repetitive sequences based on resolution-enhanced 5D experiments
JF - Journal of Biomolecular NMR
VL - 48
IS - 3
SP - 169-177
EP - 169-177
PB - Springer Netherlands
SN - 09252738
KW - Unfolded proteins
KW - Repetitive sequence
KW - Multi-dimensional NMR
KW - Non-uniform sampling
KW - Assignment
UR - http://www.springerlink.com/content/?k=doi%3a%2810.1007%2fs10858-010-9447-3%29
N2 - A strategy for complete backbone and side-chain resonance assignment of disordered proteins with highly repetitive sequence is presented. The protocol is based on three resolution-enhanced NMR experiments: 5D HN(CA)CONH provides sequential connectivity, 5D HabCabCONH is utilized to identify amino acid types, and 5D HC(CC-TOCSY)CONH is used to assign the side-chain resonances. The improved resolution was achieved by a combination of high dimensionality and long evolution times, allowed by non-uniform sampling in the indirect dimensions. Random distribution of the data points and Sparse Multidimensional Fourier Transform processing were used. Successful application of the assignment procedure to a particularly difficult protein, delta subunit of RNA polymerase from Bacillus subtilis, is shown to prove the efficiency of the strategy. The studied protein contains a disordered C-terminal region of 81 amino acids with a highly repetitive sequence. While the conventional assignment methods completely failed due to a very small differences in chemical shifts, the presented strategy provided a complete backbone and side-chain assignment.
ER -
MOTÁČKOVÁ, Veronika, Jiří NOVÁČEK, Anna ZAWADZKA-KAZIMIERCZUK, Krzysztof KAZIMIERCZUK, Lukáš ŽÍDEK, Hana ŠANDEROVÁ, Libor KRÁSNÝ, Wiktor KOZMINSKI and Vladimír SKLENÁŘ. Strategy for complete NMR assignment of disordered proteins with highly repetitive sequences based on resolution-enhanced 5D experiments. \textit{Journal of Biomolecular NMR}. Netherlands: Springer Netherlands, 2010, vol.~48, No~3, p.~169-177. ISSN~0925-2738.
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