Automated ribotyping of lactobacilli associated with early childhood caries

ŠVEC, Pavel, Ivo SEDLÁČEK and Martina KUKLETOVÁ. Automated ribotyping of lactobacilli associated with early childhood caries. In Proceedings of the 12th – International Conference on Culture Collections (ICCC12). 2010.

Basic information

• Original name: Automated ribotyping of lactobacilli associated with early childhood caries
• Authors: ŠVEC, Pavel (203 Czech Republic, guarantor, belonging to the institution), Ivo SEDLÁČEK (203 Czech Republic, belonging to the institution) and Martina KUKLETOVÁ (203 Czech Republic, belonging to the institution).
• Edition: Proceedings of the 12th – International Conference on Culture Collections (ICCC12), 2010.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Brazil
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• RIV identification code: RIV/00216224:14310/10:00045192
• Organization unit: Faculty of Science
• Keywords in English: automated ribotyping; early childhood caries; identification; Lactobacillus; RiboPrinter

Abstract

A series of 67 well-identified Lactobacillus spp. strains isolated from early childhood caries was characterised by automated ribotyping with EcoRI restriction enzyme using the RiboPrinter Microbial Characterization System (DuPont Qualicon, USA) in order to evaluate this technique for characterization and identification of lactobacilli associated with dental caries. Analysed group was identified using extensive biotyping and rep-PCR with the (GTG)5 primer and consisted of Lactobacillus fermentum (20 strains), Lactobacillus rhamnosus (15 strains), Lactobacillus casei/paracasei (14 strains), Lactobacillus gasseri (7 strains), Lactobacillus salivarius (7 strains), and Lactobacillus plantarum (4 strains). Automated ribotyping revealed 55 riboprint patterns among the analysed group. The fingerprint profiles generated by individual species were distributed into one to four clusters. The automatic identification performed by the RiboPrinter system assigned only 18 strains to the species level, remaining strains were not identified because they did not reach the 85 % similarity threshold with reference riboprint patterns . Further clustering of obtained ribotype patterns into individual groups was performed by Bionumerics v 6.0 software (Applied-Maths, Belgium) and generally corresponded with the species assignment of analysed strains. In conclusion, automated ribotyping revealed low successfulness of identification, but further cluster analysis and visual examination of obtained riboprints improved applicability of this method for the species identification of lactobacilli associated with early childhood caries. The present study was supported by the Czech Science Foundation (project 310/09/0657) and the Ministry of Education, Youth and Sports of the Czech Republic (project 1M0528).

Links

• GA310/09/0657, research and development project: Name: Molekulární taxonomie grampozitivních bakterií izolovaných ze zubního kazu v dočasné dentici

Investor: Czech Science Foundation, Molecular taxonomy of Gram-positive bacteria isolated from early childhood caries

• 1M0528, research and development project: Name: Stomatologické výzkumné centrum

Investor: Ministry of Education, Youth and Sports of the CR, Dental research centre