| TASLEROVÁ, Renata, Stanislav KOZUBEK, Eva BÁRTOVÁ, Pavla GAJDUŠKOVÁ, Roman KODET and Michal KOZUBEK. Localization of genetic elements of intact and derivative chromosome 11 and 22 territories in nuclei of Ewing sarcoma cells. Journal of Structural Biology. San Diego,USA: Academic Press, 2006, vol. 155, No 3, p. 493-504. ISSN 1047-8477. |
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@article{904676,
author = {Taslerová, Renata and Kozubek, Stanislav and Bártová, Eva and Gajdušková, Pavla and Kodet, Roman and Kozubek, Michal},
article_location = {San Diego,USA},
article_number = {3},
keywords = {HIGH-RESOLUTION CYTOMETRY; HUMAN LEUKEMIC-CELLS; HUMAN-LYMPHOCYTES; EXCHANGE ABERRATIONS; SPATIAL-ORGANIZATION; INTERPHASE NUCLEUS; BCR GENES; DOMAINS; CYCLE; TOPOGRAPHY},
language = {eng},
issn = {1047-8477},
journal = {Journal of Structural Biology},
title = {Localization of genetic elements of intact and derivative chromosome 11 and 22 territories in nuclei of Ewing sarcoma cells},
volume = {155},
year = {2006}
}
TY - JOUR
ID - 904676
AU - Taslerová, Renata - Kozubek, Stanislav - Bártová, Eva - Gajdušková, Pavla - Kodet, Roman - Kozubek, Michal
PY - 2006
TI - Localization of genetic elements of intact and derivative chromosome 11 and 22 territories in nuclei of Ewing sarcoma cells
JF - Journal of Structural Biology
VL - 155
IS - 3
SP - 493-504
EP - 493-504
PB - Academic Press
SN - 10478477
KW - HIGH-RESOLUTION CYTOMETRY
KW - HUMAN LEUKEMIC-CELLS
KW - HUMAN-LYMPHOCYTES
KW - EXCHANGE ABERRATIONS
KW - SPATIAL-ORGANIZATION
KW - INTERPHASE NUCLEUS
KW - BCR GENES
KW - DOMAINS
KW - CYCLE
KW - TOPOGRAPHY
N2 - Recurring chromosomal abnormalities are associated with specific tumour types. The EWSR1 and FLI1 genes are involved in balanced translocation t(11;22)(q24;q12), which is present in more than 85% of Ewing sarcomas. In our previous study, we have found that the fusion genes pertaining to both derivative chromosomes 11 and 22 in Ewing sarcoma cell nuclei are shifted to the midway nuclear position between the native EWSR1 and FLI1 genes. In this contribution we focused our attention at nuclear positioning of other genetic elements of chromosomes 11 and 22 in order to find if the whole derivative chromosomes or only their translocated parts change their nuclear positions in comparison with the native chromosomes. Using repeated fluorescence in situ hybridization and high-resolution cytometry, 2D radial positions of EWSR1, BCR, FLI1, BCL1 genes and fluorescence weight centres of chromosome territories were compared for intact and derivative chromosomes 11 and 22 in nuclei of three Ewing sarcoma samples. Significant radial shift was obtained for the derivative EWSR1, FLI1 and BCL1 genes and for the derivative chromosome 11 compared with the intact ones and not very significant for chromosome 22 and the BCR gene. Our results also suggest that the mean nuclear positions of fusion genes are determined by the final structure of the derivative chromosomes and do not depend on the location of the translocation event. (c) 2006 Elsevier Inc. All rights reserved.
ER -
TASLEROVÁ, Renata, Stanislav KOZUBEK, Eva BÁRTOVÁ, Pavla GAJDUŠKOVÁ, Roman KODET and Michal KOZUBEK. Localization of genetic elements of intact and derivative chromosome 11 and 22 territories in nuclei of Ewing sarcoma cells. \textit{Journal of Structural Biology}. San Diego,USA: Academic Press, 2006, vol.~155, No~3, p.~493-504. ISSN~1047-8477.
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