Detailed Information on Publication Record
2006
Localization of genetic elements of intact and derivative chromosome 11 and 22 territories in nuclei of Ewing sarcoma cells
TASLEROVÁ, Renata, Stanislav KOZUBEK, Eva BÁRTOVÁ, Pavla GAJDUŠKOVÁ, Roman KODET et. al.Basic information
Original name
Localization of genetic elements of intact and derivative chromosome 11 and 22 territories in nuclei of Ewing sarcoma cells
Authors
TASLEROVÁ, Renata (203 Czech Republic), Stanislav KOZUBEK (203 Czech Republic, guarantor), Eva BÁRTOVÁ (203 Czech Republic), Pavla GAJDUŠKOVÁ (203 Czech Republic), Roman KODET (203 Czech Republic) and Michal KOZUBEK (203 Czech Republic)
Edition
Journal of Structural Biology, San Diego,USA, Academic Press, 2006, 1047-8477
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 3.496
RIV identification code
RIV/00216224:14330/06:00040618
Organization unit
Faculty of Informatics
UT WoS
000241168000009
Keywords in English
HIGH-RESOLUTION CYTOMETRY; HUMAN LEUKEMIC-CELLS; HUMAN-LYMPHOCYTES; EXCHANGE ABERRATIONS; SPATIAL-ORGANIZATION; INTERPHASE NUCLEUS; BCR GENES; DOMAINS; CYCLE; TOPOGRAPHY
Tags
Tags
International impact, Reviewed
Změněno: 30/10/2010 11:09, prof. RNDr. Michal Kozubek, Ph.D.
Abstract
V originále
Recurring chromosomal abnormalities are associated with specific tumour types. The EWSR1 and FLI1 genes are involved in balanced translocation t(11;22)(q24;q12), which is present in more than 85% of Ewing sarcomas. In our previous study, we have found that the fusion genes pertaining to both derivative chromosomes 11 and 22 in Ewing sarcoma cell nuclei are shifted to the midway nuclear position between the native EWSR1 and FLI1 genes. In this contribution we focused our attention at nuclear positioning of other genetic elements of chromosomes 11 and 22 in order to find if the whole derivative chromosomes or only their translocated parts change their nuclear positions in comparison with the native chromosomes. Using repeated fluorescence in situ hybridization and high-resolution cytometry, 2D radial positions of EWSR1, BCR, FLI1, BCL1 genes and fluorescence weight centres of chromosome territories were compared for intact and derivative chromosomes 11 and 22 in nuclei of three Ewing sarcoma samples. Significant radial shift was obtained for the derivative EWSR1, FLI1 and BCL1 genes and for the derivative chromosome 11 compared with the intact ones and not very significant for chromosome 22 and the BCR gene. Our results also suggest that the mean nuclear positions of fusion genes are determined by the final structure of the derivative chromosomes and do not depend on the location of the translocation event. (c) 2006 Elsevier Inc. All rights reserved.
Links
| GA202/04/0907, research and development project |
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| IAA5004306, research and development project |
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| LC535, research and development project |
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| MSM 143300002, plan (intention) |
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| 1A8241, research and development project |
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