Detailed Information on Publication Record
2006
Directional motion of foreign plasmid DNA to nuclear HP1 foci
ONDŘEJ, Vladan, Stanislav KOZUBEK, Emilie LUKÁŠOVÁ, Martin FALK, Pavel MATULA et. al.Basic information
Original name
Directional motion of foreign plasmid DNA to nuclear HP1 foci
Authors
ONDŘEJ, Vladan (203 Czech Republic), Stanislav KOZUBEK (203 Czech Republic, guarantor, belonging to the institution), Emilie LUKÁŠOVÁ (203 Czech Republic), Martin FALK (203 Czech Republic), Pavel MATULA (203 Czech Republic, belonging to the institution), Petr MATULA (203 Czech Republic, belonging to the institution) and Michal KOZUBEK (203 Czech Republic, belonging to the institution)
Edition
Chromosome Research, Dordrecht, Springer, 2006, 0967-3849
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
Netherlands
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 3.057
RIV identification code
RIV/00216224:14330/06:00040619
Organization unit
Faculty of Informatics
UT WoS
000238847300003
Keywords in English
heterochromatin; HP1 protein; nuclear compartments; plasmid; transfection
Tags
International impact, Reviewed
Změněno: 24/8/2012 14:20, doc. RNDr. Martin Falk, Ph.D.
Abstract
V originále
Movement of labelled plasmid DNA relative to heterochromatin foci in nuclei, visualized with HP1-GFP, was studied using live-cell imaging and object tracking. In addition to Brownian motion of plasmid DNA we found a pronounced, non-random movement of plasmid DNA towards the nearest HP1 focus, while time-lapse microscopy showed that HP1 foci are relatively immobile and positionally stable. The movement of plasmid DNA was much faster than that of the HP1 foci. Contact of transgene DNA with an HP1 focus usually resulted in cessation of the directional motion. Moreover, the motion of plasmid DNA inside the heterochromatin compartment was more restricted (limited to 0.25 mu m) than when the plasmid DNA was outside heterochromatin (R = 0.7 mu m). Three days after transfection most of the foreign labelled DNA colocalized with centromeric heterochromatin.
Links
| GA202/02/0804, research and development project |
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| IAA1065203, research and development project |
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