| ONDŘEJ, Vladan, Stanislav KOZUBEK, Emilie LUKÁŠOVÁ, Martin FALK, Pavel MATULA, Petr MATULA and Michal KOZUBEK. Directional motion of foreign plasmid DNA to nuclear HP1 foci. Chromosome Research. Dordrecht: Springer, 2006, vol. 14, No 5, p. 505-514. ISSN 0967-3849. |
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@article{904678,
author = {Ondřej, Vladan and Kozubek, Stanislav and Lukášová, Emilie and Falk, Martin and Matula, Pavel and Matula, Petr and Kozubek, Michal},
article_location = {Dordrecht},
article_number = {5},
keywords = {heterochromatin; HP1 protein; nuclear compartments; plasmid; transfection},
language = {eng},
issn = {0967-3849},
journal = {Chromosome Research},
title = {Directional motion of foreign plasmid DNA to nuclear HP1 foci},
volume = {14},
year = {2006}
}
TY - JOUR
ID - 904678
AU - Ondřej, Vladan - Kozubek, Stanislav - Lukášová, Emilie - Falk, Martin - Matula, Pavel - Matula, Petr - Kozubek, Michal
PY - 2006
TI - Directional motion of foreign plasmid DNA to nuclear HP1 foci
JF - Chromosome Research
VL - 14
IS - 5
SP - 505-514
EP - 505-514
PB - Springer
SN - 09673849
KW - heterochromatin
KW - HP1 protein
KW - nuclear compartments
KW - plasmid
KW - transfection
N2 - Movement of labelled plasmid DNA relative to heterochromatin foci in nuclei, visualized with HP1-GFP, was studied using live-cell imaging and object tracking. In addition to Brownian motion of plasmid DNA we found a pronounced, non-random movement of plasmid DNA towards the nearest HP1 focus, while time-lapse microscopy showed that HP1 foci are relatively immobile and positionally stable. The movement of plasmid DNA was much faster than that of the HP1 foci. Contact of transgene DNA with an HP1 focus usually resulted in cessation of the directional motion. Moreover, the motion of plasmid DNA inside the heterochromatin compartment was more restricted (limited to 0.25 mu m) than when the plasmid DNA was outside heterochromatin (R = 0.7 mu m). Three days after transfection most of the foreign labelled DNA colocalized with centromeric heterochromatin.
ER -
ONDŘEJ, Vladan, Stanislav KOZUBEK, Emilie LUKÁŠOVÁ, Martin FALK, Pavel MATULA, Petr MATULA and Michal KOZUBEK. Directional motion of foreign plasmid DNA to nuclear HP1 foci. \textit{Chromosome Research}. Dordrecht: Springer, 2006, vol.~14, No~5, p.~505-514. ISSN~0967-3849.
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