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@proceedings{906935,
author = {Vařecha, Miroslav and Páclová, Daniela and Procházková, Jiřina and Matula, Pavel and Kozubek, Michal},
booktitle = {Advances in Molecular and Cancer Bilogy},
keywords = {AIF; shRNA; gene silencing; image analysis; superoxide; mitochondria; oxidative phosphorylation},
language = {eng},
isbn = {978-80-210-5312-0},
title = {Alteration in mitochondrial superoxide production by shRNA gene silencing of AIF in various cell line},
year = {2010}
}
TY - CONF
ID - 906935
AU - Vařecha, Miroslav - Páclová, Daniela - Procházková, Jiřina - Matula, Pavel - Kozubek, Michal
PY - 2010
TI - Alteration in mitochondrial superoxide production by shRNA gene silencing of AIF in various cell line
SN - 9788021053120
KW - AIF
KW - shRNA
KW - gene silencing
KW - image analysis
KW - superoxide
KW - mitochondria
KW - oxidative phosphorylation
N2 - Apart from this function in cell death, AIF also plays largely unknown role in cell life. We used AIF gene silencing using shRNA vectors followed by measurements by flow cytometry and confocal fluorescent microscopy to detect the superoxide production during oxidative phosphorylation in mitochondria. We managed to knock-down the expression of gene AIF by transient or stable transfection in various human cell lines. Interestingly, mitochondrial superoxide production in various cell lines reacted to gene silencing of AIF differently. Cell line U-2 OS transiently and also stably transfected with vector shRNA was found to produce less superoxide in comparison to control U-2 OS cells. Interestingly, HeLa cell line shows opposite results with significantly increased mitochondrial superoxide production in silenced HeLa cells. We are also in process of examination of other healthy and cancer cell. Conclusions about possible role of AIF in mitochondria based on our results with various AIF-silenced cell lines will be presented.
ER -
VAŘECHA, Miroslav, Daniela PÁCLOVÁ, Jiřina PROCHÁZKOVÁ, Pavel MATULA a Michal KOZUBEK. Alteration in mitochondrial superoxide production by shRNA gene silencing of AIF in various cell line. In \textit{Advances in Molecular and Cancer Bilogy}. ISBN~978-80-210-5312-0. 2010.
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