| TESAŘOVÁ, Lenka, Irena KRONTORÁD KOUTNÁ, Zdeněk KOŘÍSTEK and Martin KLABUSAY. Multiple myeloma patients at peripheral blood stem cell harvest: restricted usage of TCR beta variable families. Clinical Immunology. Elsevier, 2011, vol. 138, No 1, p. 67-76. ISSN 1521-6616. Available from: https://dx.doi.org/10.1016/j.clim.2010.09.007. |
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@article{907729,
author = {Tesařová, Lenka and Krontorád Koutná, Irena and Kořístek, Zdeněk and Klabusay, Martin},
article_number = {1},
doi = {http://dx.doi.org/10.1016/j.clim.2010.09.007},
keywords = {T-cell receptor; Beta variable families; Multiple myeloma; Reverse transcription polymerase chain reaction; Capillary electrophoresis},
language = {eng},
issn = {1521-6616},
journal = {Clinical Immunology},
title = {Multiple myeloma patients at peripheral blood stem cell harvest: restricted usage of TCR beta variable families},
volume = {138},
year = {2011}
}
TY - JOUR
ID - 907729
AU - Tesařová, Lenka - Krontorád Koutná, Irena - Kořístek, Zdeněk - Klabusay, Martin
PY - 2011
TI - Multiple myeloma patients at peripheral blood stem cell harvest: restricted usage of TCR beta variable families
JF - Clinical Immunology
VL - 138
IS - 1
SP - 67-76
EP - 67-76
PB - Elsevier
SN - 15216616
KW - T-cell receptor
KW - Beta variable families
KW - Multiple myeloma
KW - Reverse transcription polymerase chain reaction
KW - Capillary electrophoresis
N2 - The immune systems of multiple myeloma patients are suppressed by the disease itself, and this immunosuppression is further enhanced by standard therapies. The aim of our study was to evaluate the effects of initial chemotherapy and a peripheral blood mobilisation regimen on T-cell population diversity. Reverse transcription polymerase chain reaction (RT-PCR) with a new set of primers, in combination with capillary electrophoresis, was established. The methodology was used to analyse the relative expression of 27 T-cell receptor beta variable gene families (BV families) in multiple myeloma patients undergoing peripheral blood stem cell harvest. We found that the overall BV family usage in these patients was restricted; the relative expression of 10 BV families was significantly depressed in patients compared to healthy donors. These findings demonstrate that the preparative regimen for autologous stem cell transplantation affects the T-cell population in terms of the restriction of its T-cell receptor diversity.
ER -
TESAŘOVÁ, Lenka, Irena KRONTORÁD KOUTNÁ, Zdeněk KOŘÍSTEK and Martin KLABUSAY. Multiple myeloma patients at peripheral blood stem cell harvest: restricted usage of TCR beta variable families. \textit{Clinical Immunology}. Elsevier, 2011, vol.~138, No~1, p.~67-76. ISSN~1521-6616. Available from: https://dx.doi.org/10.1016/j.clim.2010.09.007.
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