| POTĚŠILOVÁ, Michaela, Miroslav VAŘECHA, Lenka TESAŘOVÁ and Irena KRONTORÁD KOUTNÁ. Time lapse living cells microscopy for studying CML: Preparation of Abl1- and Bcr/Abl - fluorescent protein vectors using Gateway technology. In Advances in Molecular and Cancer Biology, 2010. Brno: muni PRESS, 2010, p. 31-35. ISBN 978-80-210-5312-0. |
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@inproceedings{907855,
author = {Potěšilová, Michaela and Vařecha, Miroslav and Tesařová, Lenka and Krontorád Koutná, Irena},
address = {Brno},
booktitle = {Advances in Molecular and Cancer Biology, 2010},
keywords = {Gateway cloning ABL1 BCR/ABL expression vectors},
language = {eng},
location = {Brno},
isbn = {978-80-210-5312-0},
pages = {31-35},
publisher = {muni PRESS},
title = {Time lapse living cells microscopy for studying CML: Preparation of Abl1- and Bcr/Abl - fluorescent protein vectors using Gateway technology},
year = {2010}
}
TY - JOUR
ID - 907855
AU - Potěšilová, Michaela - Vařecha, Miroslav - Tesařová, Lenka - Krontorád Koutná, Irena
PY - 2010
TI - Time lapse living cells microscopy for studying CML: Preparation of Abl1- and Bcr/Abl - fluorescent protein vectors using Gateway technology
PB - muni PRESS
CY - Brno
SN - 9788021053120
KW - Gateway cloning ABL1 BCR/ABL expression vectors
N2 - Using time-lapse microscopy, protein localization and dynamics can be studied directly in living cells. This may bring significant information necessary to complete understanding of protein function. Aberrant tyrosinkinase Bcr/Abl is the main cause of chronic myelogenous leukemia. Abl1 tyrosinkinase represents its native form. Among others these natural and aberrant kinase differ in cell localization, which will be the subject for subsequent examination in the K562 leukemic cell line or in CD34+ cells from bone marrow. We created a unique set of Bcr/Abl and Abl1 expression vectors tagged with EYFP or RFP sequences for examination of protein pools movements in the living cells. The Gateway cloning system was chosen for its flexibility and robustness. We applied two types of fluorescent protein to be able watch both forms of kinase simultaneously.
ER -
POTĚŠILOVÁ, Michaela, Miroslav VAŘECHA, Lenka TESAŘOVÁ and Irena KRONTORÁD KOUTNÁ. Time lapse living cells microscopy for studying CML: Preparation of Abl1- and Bcr/Abl - fluorescent protein vectors using Gateway technology. In \textit{Advances in Molecular and Cancer Biology, 2010}. Brno: muni PRESS, 2010, p.~31-35. ISBN~978-80-210-5312-0.
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