Dexamethasone and Insulin Affecting SFTPA1 and SFTPA2 mRNA
Levels

D 2010

Dexamethasone and Insulin Affecting SFTPA1 and SFTPA2 mRNA Levels

RUČKA, Zdeněk, Lenka TESAŘOVÁ, Michaela POTĚŠILOVÁ, Stanislav STEJSKAL, Irena KRONTORÁD KOUTNÁ et. al.

Basic information

Original name

Dexamethasone and Insulin Affecting SFTPA1 and SFTPA2 mRNA Levels

Authors

RUČKA, Zdeněk (203 Czech Republic, belonging to the institution), Lenka TESAŘOVÁ (203 Czech Republic, belonging to the institution), Michaela POTĚŠILOVÁ (203 Czech Republic, belonging to the institution), Stanislav STEJSKAL (203 Czech Republic, belonging to the institution), Irena KRONTORÁD KOUTNÁ (203 Czech Republic, guarantor, belonging to the institution), Petr VAŇHARA (203 Czech Republic, belonging to the institution) and Jan DOLEŽEL (203 Czech Republic, belonging to the institution)

Edition

Brno, Česká republika, Advances in Molecular and Cancer Biology, 2010, p. 36-38, 3 pp. 2010

Publisher

muni PRESS

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14330/10:00045383

Organization unit

Faculty of Informatics

ISBN

978-80-210-5312-0

Keywords in English

Lung surfactant; Insulin; Dexamethasone

Abstract

V originále

Lung surfactants primary function is to lower surface tension and therefore it protects alveoli from collapsing. It consists of phospholipids and four proteins. These proteins stabilize the surfactant layer (SFTPB and SFTPC) and modulate immunological response to various pathogens (SFTPA, SFTPD), which is the secondary role of lung surfactant. Low levels of surfactant proteins lead to severe diseases. Dexamethasone and insulin were applied on H441 cell line. This cell line resembles Clara cells and therefore serves as a model of lung surfactant producing cells. SFTPA1 (and its analog SFTPA2) were not affected by insulin (2500 ng/mL), but dexamethasone treatment (10-7 M) for 48 hours resulted in 50% decrease in their mRNA production. Surprisingly, combination of dexamethasone and insulin resulted in 90% decrease.

Links

NS9681, research and development project

Name: Studium molekulárně-biologické odpovědi na intenzivní inzulínovou terapii při plicních resekcích

Investor: Ministry of Health of the CR

Citovat

RUČKA, Zdeněk, Lenka TESAŘOVÁ, Michaela POTĚŠILOVÁ, Stanislav STEJSKAL, Irena KRONTORÁD KOUTNÁ, Petr VAŇHARA and Jan DOLEŽEL. Dexamethasone and Insulin Affecting SFTPA1 and SFTPA2 mRNA Levels. In Advances in Molecular and Cancer Biology, 2010. Brno, Česká republika: muni PRESS, 2010, p. 36-38. ISBN 978-80-210-5312-0.

@inproceedings{907864,
   author = {Ručka, Zdeněk and Tesařová, Lenka and Potěšilová, Michaela and Stejskal, Stanislav and Krontorád Koutná, Irena and Vaňhara, Petr and Doležel, Jan},
   address = {Brno, Česká republika},
   booktitle = {Advances in Molecular and Cancer Biology, 2010},
   keywords = {Lung surfactant; Insulin; Dexamethasone},
   language = {eng},
   location = {Brno, Česká republika},
   isbn = {978-80-210-5312-0},
   pages = {36-38},
   publisher = {muni PRESS},
   title = {Dexamethasone and Insulin Affecting SFTPA1 and SFTPA2 mRNA Levels},
   year = {2010}
}

TY  - JOUR
ID  - 907864
AU  - Ručka, Zdeněk - Tesařová, Lenka - Potěšilová, Michaela - Stejskal, Stanislav - Krontorád Koutná, Irena - Vaňhara, Petr - Doležel, Jan
PY  - 2010
TI  - Dexamethasone and Insulin Affecting SFTPA1 and SFTPA2 mRNA Levels
PB  - muni PRESS
CY  - Brno, Česká republika
SN  - 9788021053120
KW  - Lung surfactant
KW  - Insulin
KW  - Dexamethasone
N2  - Lung surfactants primary function is to lower surface tension and therefore it protects alveoli from collapsing. It consists of phospholipids and four proteins. These proteins stabilize the surfactant layer (SFTPB and SFTPC) and modulate immunological response to various pathogens (SFTPA, SFTPD), which is the secondary role of lung surfactant. Low levels of surfactant proteins lead to severe diseases. Dexamethasone and insulin were applied on H441 cell line. This cell line resembles Clara cells and therefore serves as a model of lung surfactant producing cells. SFTPA1 (and its analog SFTPA2) were not affected by insulin (2500 ng/mL), but dexamethasone treatment (10-7 M) for 48 hours resulted in 50% decrease in their mRNA production. Surprisingly, combination of dexamethasone and insulin resulted in 90% decrease.
ER  -

RUČKA, Zdeněk, Lenka TESAŘOVÁ, Michaela POTĚŠILOVÁ, Stanislav STEJSKAL, Irena KRONTORÁD KOUTNÁ, Petr VAŇHARA and Jan DOLEŽEL. Dexamethasone and Insulin Affecting SFTPA1 and SFTPA2 mRNA Levels. In \textit{Advances in Molecular and Cancer Biology, 2010}. Brno, Česká republika: muni PRESS, 2010, p.~36-38. ISBN~978-80-210-5312-0.

Detailed Information on Publication Record