RUČKA, Zdeněk, Lenka TESAŘOVÁ, Michaela POTĚŠILOVÁ, Stanislav STEJSKAL, Irena KRONTORÁD KOUTNÁ, Petr VAŇHARA and Jan DOLEŽEL. Dexamethasone and Insulin Affecting SFTPA1 and SFTPA2 mRNA Levels. In Advances in Molecular and Cancer Biology, 2010. Brno, Česká republika: muni PRESS, 2010, p. 36-38. ISBN 978-80-210-5312-0.
Other formats:   BibTeX LaTeX RIS
Basic information
Original name Dexamethasone and Insulin Affecting SFTPA1 and SFTPA2 mRNA Levels
Authors RUČKA, Zdeněk (203 Czech Republic, belonging to the institution), Lenka TESAŘOVÁ (203 Czech Republic, belonging to the institution), Michaela POTĚŠILOVÁ (203 Czech Republic, belonging to the institution), Stanislav STEJSKAL (203 Czech Republic, belonging to the institution), Irena KRONTORÁD KOUTNÁ (203 Czech Republic, guarantor, belonging to the institution), Petr VAŇHARA (203 Czech Republic, belonging to the institution) and Jan DOLEŽEL (203 Czech Republic, belonging to the institution).
Edition Brno, Česká republika, Advances in Molecular and Cancer Biology, 2010, p. 36-38, 3 pp. 2010.
Publisher muni PRESS
Other information
Original language English
Type of outcome Proceedings paper
Field of Study Genetics and molecular biology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14330/10:00045383
Organization unit Faculty of Informatics
ISBN 978-80-210-5312-0
Keywords in English Lung surfactant; Insulin; Dexamethasone
Tags cbia-web
Tags Reviewed
Changed by Changed by: Mgr. Lenka Tesařová, Ph.D., učo 78340. Changed: 31/10/2011 09:02.
Abstract
Lung surfactants primary function is to lower surface tension and therefore it protects alveoli from collapsing. It consists of phospholipids and four proteins. These proteins stabilize the surfactant layer (SFTPB and SFTPC) and modulate immunological response to various pathogens (SFTPA, SFTPD), which is the secondary role of lung surfactant. Low levels of surfactant proteins lead to severe diseases. Dexamethasone and insulin were applied on H441 cell line. This cell line resembles Clara cells and therefore serves as a model of lung surfactant producing cells. SFTPA1 (and its analog SFTPA2) were not affected by insulin (2500 ng/mL), but dexamethasone treatment (10-7 M) for 48 hours resulted in 50% decrease in their mRNA production. Surprisingly, combination of dexamethasone and insulin resulted in 90% decrease.
Links
NS9681, research and development projectName: Studium molekulárně-biologické odpovědi na intenzivní inzulínovou terapii při plicních resekcích
Investor: Ministry of Health of the CR
PrintDisplayed: 21/7/2024 11:54