Detailed Information on Publication Record
2011
Proliferation and differentiation potential of CD133+ and CD34+ populations from the bone marrow and mobilized peripheral blood
KRONTORÁD KOUTNÁ, Irena, Martina PETERKOVÁ, Pavel ŠIMARA, Stanislav STEJSKAL, Lenka TESAŘOVÁ et. al.Basic information
Original name
Proliferation and differentiation potential of CD133+ and CD34+ populations from the bone marrow and mobilized peripheral blood
Name in Czech
Proliferační a diferenciační potenciál CD133+ a CD34+ buněčných populací kostní dřeně a periferní mobilizované krve
Authors
KRONTORÁD KOUTNÁ, Irena (203 Czech Republic, guarantor, belonging to the institution), Martina PETERKOVÁ (203 Czech Republic, belonging to the institution), Pavel ŠIMARA (203 Czech Republic, belonging to the institution), Stanislav STEJSKAL (203 Czech Republic, belonging to the institution), Lenka TESAŘOVÁ (203 Czech Republic, belonging to the institution) and Michal KOZUBEK (203 Czech Republic, belonging to the institution)
Edition
Annals of hematology, Germany, Springer, 2011, 0939-5555
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30200 3.2 Clinical medicine
Country of publisher
Germany
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.615
RIV identification code
RIV/00216224:14330/11:00051523
Organization unit
Faculty of Informatics
UT WoS
000286053500001
Keywords (in Czech)
CD34 ;CD133; HSC; transplantace; VEGFR
Keywords in English
CD34; CD133; HSC; transplantation; VEGFR
Tags
Tags
International impact, Reviewed
Změněno: 13/4/2011 12:53, doc. RNDr. Irena Koutná, Ph.D.
Abstract
V originále
CD34 is the most frequently used marker for the selection of cells for bone marrow (BM) transplantation. The use of CD133 as an alternative marker is an open research topic. The goal of this study was to evaluate the proliferation and differentiation potential for hematopoiesis (short and long term) of CD133+ and CD34+ populations from bone marrow and mobilized peripheral blood. Eight cell populations were compared: CD34+ and CD133+ cells from both the BM (CML Ph-, CML Ph+, and healthy volunteers) and mobilized peripheral blood cells. Multicolor flow cytometry and cultivation experiments were used to measure expression and differentiation of the individual populations. It was observed that the CD133+ BM population showed higher cell expansion. Another finding is that during a 6-day cultivation with 5(6)-carboxyfluorescein diacetate N- succinimidyl ester (CFSE), more cells remained in division D0 (non-dividing cells). There was a higher percentage of CD38- cells observed on the CD133+ BM population. It was also observed that the studied populations contained very similar but not the same pools of progenitors: erythroid, lymphoid, and myeloid. This was confirmed by CFU-GM and CFU-E experiments. The VEGFR antigen was used to monitor subpopulations of endothelial sinusoidal progenitors. The CD133+ BM population contained significantly more VEGFR+ cells. Our findings suggest that the CD133+ population from the BM shows better proliferation activity and a higher distribution of primitive progenitors than any other studied population.
Links
| MSM0021622430, plan (intention) |
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| 2B06052, research and development project |
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