J 2011

Recognition of transcription termination signal by the nuclear polyadenylated RNA-binding (Nab)3 protein

HÓBOR, Fruzsina, Roberto PERGOLI, Karel KUBÍČEK, Dominika HROŠŠOVÁ, Veronika BAČÍKOVÁ et. al.

Basic information

Original name

Recognition of transcription termination signal by the nuclear polyadenylated RNA-binding (Nab)3 protein

Authors

HÓBOR, Fruzsina (348 Hungary, belonging to the institution), Roberto PERGOLI (380 Italy, belonging to the institution), Karel KUBÍČEK (203 Czech Republic, belonging to the institution), Dominika HROŠŠOVÁ (703 Slovakia, belonging to the institution), Veronika BAČÍKOVÁ (203 Czech Republic, belonging to the institution), Michal ZIMMERMANN (203 Czech Republic, belonging to the institution), Josef PASULKA (203 Czech Republic, belonging to the institution), Ctirad HOFR (203 Czech Republic, belonging to the institution), Štěpánka VAŇÁČOVÁ (203 Czech Republic, belonging to the institution) and Richard ŠTEFL (203 Czech Republic, guarantor, belonging to the institution)

Edition

The Journal of Biological Chemistry, Am. Soc. for Biochem. and Mol. Biol. 2011, 0021-9258

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 4.773

RIV identification code

RIV/00216224:14740/11:00049398

Organization unit

Central European Institute of Technology

DOI

UT WoS

000286653200048

Keywords (in Czech)

NMR; proteinová struktura; zpracování RNA; interakce mezi proteinem a RNA; ukončení transkripce

Keywords in English

NMR; Protein structure; RNA processing; RNA-protein interaction; Transcription termination

Tags

Tags

International impact, Reviewed
Změněno: 25/3/2012 05:35, Olga Křížová

Abstract

ORIG CZ

V originále

Non-coding RNA polymerase II transcripts are processed by the poly(A) independent termination pathway that requires the Nrd1 complex. The Nrd1 complex includes two RNA-binding proteins, the nuclear polyadenylated RNA-binding (Nab)3 and the nuclear pre-mRNA down-regulation (Nrd)1 that bind their specific termination elements. Here we report the solution structure of the RNA-recognition motif (RRM) of Nab3 in complex with a UCUU oligonucleotide, representing the Nab3 termination element. The structure shows that the first three nucleotides of UCUU are accommodated on the beta-sheet surface of Nab3 RRM, but reveals a sequence specific recognition only for the central cytidine and uridine. The specific contacts we identified are important for binding affinity in vitro as well as for yeast viability. Further, we show that both RNA-binding motifs of Nab3 and Nrd1 alone bind their termination elements with a weak affinity. Interestingly, when Nab3 and Nrd1 form a heterodimer, the affinity to RNA is significantly increased due to the cooperative binding. These findings are in accordance with the model of their function in the poly(A) independent termination, in which the binding to the combined and/or repetitive termination elements elicits efficient termination.

In Czech

Článek se zabývá studiem struktury a biologické funkce RNA vázajícího proteinu Nab3 a jeho interakce s oligonukleotidem UCUU.

Links

GAP305/10/1490, research and development project
Name: Strukturní podstata ukončení transkripce nezávislé na poly(A) signálu
Investor: Czech Science Foundation
GA204/08/1212, research and development project
Name: Strukturní studium interakcí mezi proteiny a RNA účastnící se v mechanismu kontroly kvality RNA
Investor: Czech Science Foundation, Structural studies of protein-RNA complexes involved in RNA quality control
GD204/08/H054, research and development project
Name: Molekulární mechanismy proliferace a diferenciace buněk
Investor: Czech Science Foundation, Molecular mechanisms of the cell proliferation and differentiation
IAA401630903, research and development project
Name: Strukturní podstata mechanismu ukončení transkripce nepolyadenylovaných transkriptů
Investor: Academy of Sciences of the Czech Republic, Structural basis for transcription termination of nonpolyadenylated transcripts
LA08008, research and development project
Name: Strukturní studium interakcí mezi bíkovinami a poškozenou RNA.
Investor: Ministry of Education, Youth and Sports of the CR, Structural studies of protein-RNA complexes involved in RNA quality control
MSM0021622413, plan (intention)
Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment
MSM0021622415, plan (intention)
Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
084316/Z/07/Z, interní kód MU
Name: Polyadenylation and mechanisms of nuclear RNA quality control
Investor: Wellcome Trust, Polyadenylation and mechanisms of nuclear RNA quality control
1642, interní kód MU
Name: EMBO Young Investigator Programme (Acronym: EMBO)
Investor: EMBO (European Molecular Biology Organization), EMBO Young Investigator Programme
205872, interní kód MU
Name: Program developing interdisciplinary research POtential for the STudies of BIOMolecular INteractions (Acronym: POSTBIOMIN)
Investor: European Union, Program developing interdisciplinary research POtential for the STudies of BIOMolecular INteractions, Capacities