a 2010

Association between major histocompatibility complex class IIB alleles and metazoan parasite load in populations of European chub (Leuciscus cephalus): testing of balancing selection

SEIFERTOVÁ, Mária, Jiří JARKOVSKÝ, Martina VYSKOČILOVÁ and Andrea VETEŠNÍKOVÁ ŠIMKOVÁ

Basic information

Original name

Association between major histocompatibility complex class IIB alleles and metazoan parasite load in populations of European chub (Leuciscus cephalus): testing of balancing selection

Authors

SEIFERTOVÁ, Mária (703 Slovakia, guarantor, belonging to the institution), Jiří JARKOVSKÝ (203 Czech Republic, belonging to the institution), Martina VYSKOČILOVÁ (203 Czech Republic) and Andrea VETEŠNÍKOVÁ ŠIMKOVÁ (203 Czech Republic, belonging to the institution)

Edition

18th annual meeting of the society for molecular biology and evolution, 2010

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

France

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/10:00045467

Organization unit

Faculty of Science

Keywords in English

MHC-parasites-freshwater fish

Abstract

V originále

The genes of major histocompatibility complex (MHC), the most polymorphic loci known in vertebrates, have become the most studied genes in molecular ecology because they represent one of the best candidates suitable to study natural selection on a molecular level in the wild populations. The extremely high level of MHC allelic diversity is result of many mechanisms, especially the parasite-driven balancing selection, as well as recombination, gene duplication mutation and genetic drift. The main objectives of the present study were: a) to perform a sequence analysis of MHC class IIB alleles in European chub (Leuciscus cephalus), b) to investigate the contribution of evolutionary mechanisms (selection, recombination) to MHC diversity, and c) to analyse the co-evolutionary relationship between metazoan parasite load and MHC class IIB alleles. A total of 311 individuals from 15 populations of chub were collected from European rivers across a wide geographical range. The metazoan parasite load was analyzed at the levels of infracommunity and component community. In order to elucidate the participation of neutral evolutionary mechanisms on maintaining the MHC polymorphism, twelve microsatellite loci and mtDNA (cytochrome b) were analyzed. The associations between metazoan parasites and MHC class IIB alleles were analyzed by using Mantel test and co-inertia analysis (COIA). The results showed an influence of host phylogeny on composition of parasite communities and suggest the relationship between MHC allelic diversity and parasite occurrence. Co-inertia analysis revealed that several MHC alleles are associated to susceptibility to the specific metazoan parasites.

Links

GA524/07/0188, research and development project
Name: Úloha imunitní investice v kontextu kompromisů: imunoekologické studium vztahů mezi reprodukcí, imunitou a parazitizmem u sladkovodních ryb
Investor: Czech Science Foundation, The role of immune investment in the context of trade-offs: immuno-ecological study of the relationships among reproduction, immunity and parasitism
LC522, research and development project
Name: ICHTYOPARAZITOLOGIE - centrum základního výzkumu
Investor: Ministry of Education, Youth and Sports of the CR, Ichtyoparazitology - the center of basic research
MSM0021622416, plan (intention)
Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase
Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time