D 2010

SOLVING PHASE PROBLEM USING A SE-MET DERIVATIVE OF THE FLAVOENZYME NAD(P)H:ACCEPTOR OXIDOREDUCTASE (FERB)

KLUMPLER, Tomáš, Jaromír MAREK, Vojtěch SEDLÁČEK and Igor KUČERA

Basic information

Original name

SOLVING PHASE PROBLEM USING A SE-MET DERIVATIVE OF THE FLAVOENZYME NAD(P)H:ACCEPTOR OXIDOREDUCTASE (FERB)

Authors

Edition

Česká Republika, Materials Structure, vol. 17, no. 1a (2010), 3 pp. 2010

Publisher

Czech and Slovak Crystallographic Association

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Science

ISSN

Keywords in English

phase problem; flavoenzyme; FerB; reductase; crystallography
Změněno: 6/12/2010 19:02, Mgr. Tomáš Klumpler, Ph.D.

Abstract

V originále

The flavin adenine dinucleotide-dependent enzyme FerB from Paracoccus denitrificans reduces a range of sub strates, including chromate, ferric complexes, benzoquinones and naphtoquinones. The reduced form of nicotinamide adenine dinucleotide serves as a source of electrons. Recombinant unmodified and selenometionine-substituted (Se-Met) FerB derivatives were crystallized, diffraction data for both forms were collected and the phase problem for Se-Met FerB dimer was solved by three-wavelength multiple anomalous dispersion, followed by the combination of molecular replacement and single-wavelength anomalous diffraction phasing. A molecular replacement solution of unmodified FerB tetramer was obtained using Se-Met structure as a search model.

Links

GA525/07/1069, research and development project
Name: Struktura, funkce a regulace FerB, širokospecifické bakteriální oxidoreduktasy s možným významem pro ekotechnologii
Investor: Czech Science Foundation, Structure, function and regulation of FerB, a broad-specificity bacterial oxidoreductase of a potential ecotechnological relevance
GD204/08/H054, research and development project
Name: Molekulární mechanismy proliferace a diferenciace buněk
Investor: Czech Science Foundation, Molecular mechanisms of the cell proliferation and differentiation
LC06034, research and development project
Name: Regulace morfogeneze rostlinných buněk a orgánů
Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs
MSM0021622413, plan (intention)
Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment
MSM0021622415, plan (intention)
Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations