Dynamic structure and binding specifity of the receiver domain
of sensor histidine kinase CKI1

D 2010

Dynamic structure and binding specifity of the receiver domain of sensor histidine kinase CKI1

PEKÁROVÁ, Blanka, Tomáš KLUMPLER, Olga TŘÍSKOVÁ, Jakub HORÁK, Lukáš ŽÍDEK et. al.

Basic information

Original name

Dynamic structure and binding specifity of the receiver domain of sensor histidine kinase CKI1

Name in Czech

Dynamika struktury a vazebná specifita přijímačové domény senzorové histidinkinázy CKI1

Authors

PEKÁROVÁ, Blanka, Tomáš KLUMPLER, Olga TŘÍSKOVÁ, Jakub HORÁK, Lukáš ŽÍDEK, Jaromír MAREK, Radka DOPITOVÁ, Jan HEJÁTKO and Lubomír JANDA

Edition

Tarragona, Spain, p. 85-86, 2 pp. 2010

Publisher

IPGSA conference 2010

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Spain

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Organization unit

Faculty of Science

Keywords in English

multistep phosphorelay, crystal structure, NMR analysis, CKI1

Změněno: 29/4/2011 15:32, RNDr. Lubomír Janda, Ph.D.

Abstract

V originále

In the Arabidopsis two-component signaling, the signal is transfered from sensor histidine kinase via histidine-containing phosphotransfer proteins (AHP1-5) to nuclear response regulators. Here we show the receiver domain of histidine kinase CYTOKININ-INDEPENDENT1 (CKI1RD) interacts in vivo and in vitro with AHP2, 3 and 5 with different affinities. To unravel the molecular determinants of observed specificity, we determined crystal structure of free CKI1RD and CKI1RD in complex with magnesium ions. Dynamics of CKI1RD solution structure has been studied in details by NMR in absence or presence of magnesium ions and beryllium fluoride.

Links

GA521/09/1699, research and development project

Name: Imunomodulace jako nástroj funkční proteomiky při studiu cytokininové signální dráhy u Arabidopsis thaliana

Investor: Czech Science Foundation, Immunomodulation as a functional proteomics tool for cytokinin signaling study in Arabidopsis thaliana

LC06034, research and development project

Name: Regulace morfogeneze rostlinných buněk a orgánů

Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs

MSM0021622413, plan (intention)

Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment

MSM0021622415, plan (intention)

Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

Citovat

PEKÁROVÁ, Blanka, Tomáš KLUMPLER, Olga TŘÍSKOVÁ, Jakub HORÁK, Lukáš ŽÍDEK, Jaromír MAREK, Radka DOPITOVÁ, Jan HEJÁTKO and Lubomír JANDA. Dynamic structure and binding specifity of the receiver domain of sensor histidine kinase CKI1. Tarragona, Spain: IPGSA conference 2010, 2010, p. 85-86.

@inproceedings{911208,
   author = {Pekárová, Blanka and Klumpler, Tomáš and Třísková, Olga and Horák, Jakub and Žídek, Lukáš and Marek, Jaromír and Dopitová, Radka and Hejátko, Jan and Janda, Lubomír},
   address = {Tarragona, Spain},
   keywords = {multistep phosphorelay, crystal structure, NMR analysis, CKI1},
   language = {eng},
   location = {Tarragona, Spain},
   pages = {85-86},
   publisher = {IPGSA conference 2010},
   title = {Dynamic structure and binding specifity of the receiver domain of sensor histidine kinase CKI1},
   url = {http://events.emagister.co.uk/conferences/20th_ipgsa_conference__international_plant_growth_substances_association/14212},
   year = {2010}
}

TY  - JOUR
ID  - 911208
AU  - Pekárová, Blanka - Klumpler, Tomáš - Třísková, Olga - Horák, Jakub - Žídek, Lukáš - Marek, Jaromír - Dopitová, Radka - Hejátko, Jan - Janda, Lubomír
PY  - 2010
TI  - Dynamic structure and binding specifity of the receiver domain of sensor histidine kinase CKI1
PB  - IPGSA conference 2010
CY  - Tarragona, Spain
KW  - multistep phosphorelay, crystal structure, NMR analysis, CKI1
UR  - http://events.emagister.co.uk/conferences/20th_ipgsa_conference__international_plant_growth_substances_association/14212
N2  - In the Arabidopsis two-component signaling, the signal is transfered from sensor histidine kinase via histidine-containing phosphotransfer proteins (AHP1-5) to nuclear response regulators. Here we show the receiver domain of histidine kinase CYTOKININ-INDEPENDENT1 (CKI1RD) interacts in vivo and in vitro with AHP2, 3 and 5 with different affinities. To unravel the molecular determinants of observed specificity, we determined crystal structure of free CKI1RD and CKI1RD in complex with magnesium ions. Dynamics of CKI1RD solution structure has been studied in details by NMR in absence or presence of magnesium ions and beryllium fluoride.
ER  -

PEKÁROVÁ, Blanka, Tomáš KLUMPLER, Olga TŘÍSKOVÁ, Jakub HORÁK, Lukáš ŽÍDEK, Jaromír MAREK, Radka DOPITOVÁ, Jan HEJÁTKO and Lubomír JANDA. \textit{Dynamic structure and binding specifity of the receiver domain of sensor histidine kinase CKI1}. Tarragona, Spain: IPGSA conference 2010, 2010, p.~85-86.

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