Dynamic structure and binding specifity of the receiver domain of sensor histidine kinase CKI1

PEKÁROVÁ, Blanka, Tomáš KLUMPLER, Olga TŘÍSKOVÁ, Jakub HORÁK, Lukáš ŽÍDEK, Jaromír MAREK, Radka DOPITOVÁ, Jan HEJÁTKO and Lubomír JANDA. Dynamic structure and binding specifity of the receiver domain of sensor histidine kinase CKI1. Tarragona, Spain: IPGSA conference 2010, 2010, p. 85-86.

Basic information

• Original name: Dynamic structure and binding specifity of the receiver domain of sensor histidine kinase CKI1
• Name in Czech: Dynamika struktury a vazebná specifita přijímačové domény senzorové histidinkinázy CKI1
• Authors: PEKÁROVÁ, Blanka, Tomáš KLUMPLER, Olga TŘÍSKOVÁ, Jakub HORÁK, Lukáš ŽÍDEK, Jaromír MAREK, Radka DOPITOVÁ, Jan HEJÁTKO and Lubomír JANDA.
• Edition: Tarragona, Spain, p. 85-86, 2 pp. 2010.
• Publisher: IPGSA conference 2010

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Spain
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Organization unit: Faculty of Science
• Keywords in English: multistep phosphorelay, crystal structure, NMR analysis, CKI1

Abstract

In the Arabidopsis two-component signaling, the signal is transfered from sensor histidine kinase via histidine-containing phosphotransfer proteins (AHP1-5) to nuclear response regulators. Here we show the receiver domain of histidine kinase CYTOKININ-INDEPENDENT1 (CKI1RD) interacts in vivo and in vitro with AHP2, 3 and 5 with different affinities. To unravel the molecular determinants of observed specificity, we determined crystal structure of free CKI1RD and CKI1RD in complex with magnesium ions. Dynamics of CKI1RD solution structure has been studied in details by NMR in absence or presence of magnesium ions and beryllium fluoride.

Links

• GA521/09/1699, research and development project: Name: Imunomodulace jako nástroj funkční proteomiky při studiu cytokininové signální dráhy u Arabidopsis thaliana

Investor: Czech Science Foundation, Immunomodulation as a functional proteomics tool for cytokinin signaling study in Arabidopsis thaliana

• LC06034, research and development project: Name: Regulace morfogeneze rostlinných buněk a orgánů

Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs

• MSM0021622413, plan (intention): Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations