Detailed Information on Publication Record
2010
Identification of Staphylococcus spp. using (GTG)5-PCR fingerprinting
ŠVEC, Pavel, Roman PANTŮČEK, Petr PETRÁŠ, Ivo SEDLÁČEK, Dana NOVÁKOVÁ et. al.Basic information
Original name
Identification of Staphylococcus spp. using (GTG)5-PCR fingerprinting
Authors
ŠVEC, Pavel (203 Czech Republic, guarantor, belonging to the institution), Roman PANTŮČEK (203 Czech Republic, belonging to the institution), Petr PETRÁŠ (203 Czech Republic), Ivo SEDLÁČEK (203 Czech Republic, belonging to the institution) and Dana NOVÁKOVÁ (203 Czech Republic, belonging to the institution)
Edition
Systematic and Applied Microbiology, Germany, Elsevier GmbH, 2010, 0723-2020
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Germany
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 3.075
RIV identification code
RIV/00216224:14310/10:00045696
Organization unit
Faculty of Science
UT WoS
000286481200004
Keywords in English
Staphylococcus; (GTG)5-PCR; rep-PCR; rpoB gene sequencing; Identification; Taxonomy
Tags
International impact, Reviewed
Změněno: 21/3/2011 07:26, doc. RNDr. Pavel Švec, Ph.D.
Abstract
V originále
A group of 212 type and reference strains deposited in the Czech Collection of Microorganisms (Brno, Czech Republic) and covering 41 Staphylococcus species comprising 21 subspecies was characterised using rep-PCR fingerprinting with the (GTG)5 primer in order to evaluate this method for identification of staphylococci. All strains were typeable using the (GTG)5 primer and generated PCR products ranging from 200 to 4500 bp. Numerical analysis of the obtained fingerprints revealed (sub)speciesspecific clustering corresponding with the taxonomic position of analysed strains. Taxonomic position of selected strains representing the (sub)species that were distributed over multiple rep-PCR clusters was verified and confirmed by the partial rpoB gene sequencing. Staphylococcus caprae, Staphylococcus equorum, Staphylococcus sciuri, Staphylococcus piscifermentans, Staphylococcus xylosus, and Staphylococcus saprophyticus revealed heterogeneous fingerprints and each (sub)species was distributed over several clusters. However, representatives of the remaining Staphylococcus spp. were clearly separated in single (sub)species-specific clusters. These results showed rep-PCR with the (GTG)5 primer as a fast and reliable method applicable for differentiation and straightforward identification of majority of Staphylococcus spp.
Links
| GA310/09/0459, research and development project |
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| MSM0021622415, plan (intention) |
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| MSM0021622416, plan (intention) |
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