Identification of Staphylococcus spp. using (GTG)5-PCR fingerprinting

ŠVEC, Pavel, Roman PANTŮČEK, Petr PETRÁŠ, Ivo SEDLÁČEK and Dana NOVÁKOVÁ. Identification of Staphylococcus spp. using (GTG)5-PCR fingerprinting. Systematic and Applied Microbiology. Germany: Elsevier GmbH, 2010, vol. 33, No 8, p. 451-456. ISSN 0723-2020.

Basic information

• Original name: Identification of Staphylococcus spp. using (GTG)5-PCR fingerprinting
• Authors: ŠVEC, Pavel (203 Czech Republic, guarantor, belonging to the institution), Roman PANTŮČEK (203 Czech Republic, belonging to the institution), Petr PETRÁŠ (203 Czech Republic), Ivo SEDLÁČEK (203 Czech Republic, belonging to the institution) and Dana NOVÁKOVÁ (203 Czech Republic, belonging to the institution).
• Edition: Systematic and Applied Microbiology, Germany, Elsevier GmbH, 2010, 0723-2020.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Germany
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 3.075
• RIV identification code: RIV/00216224:14310/10:00045696
• Organization unit: Faculty of Science
• UT WoS: 000286481200004
• Keywords in English: Staphylococcus; (GTG)5-PCR; rep-PCR; rpoB gene sequencing; Identification; Taxonomy
• Tags: International impact, Reviewed

Abstract

A group of 212 type and reference strains deposited in the Czech Collection of Microorganisms (Brno, Czech Republic) and covering 41 Staphylococcus species comprising 21 subspecies was characterised using rep-PCR fingerprinting with the (GTG)5 primer in order to evaluate this method for identification of staphylococci. All strains were typeable using the (GTG)5 primer and generated PCR products ranging from 200 to 4500 bp. Numerical analysis of the obtained fingerprints revealed (sub)speciesspecific clustering corresponding with the taxonomic position of analysed strains. Taxonomic position of selected strains representing the (sub)species that were distributed over multiple rep-PCR clusters was verified and confirmed by the partial rpoB gene sequencing. Staphylococcus caprae, Staphylococcus equorum, Staphylococcus sciuri, Staphylococcus piscifermentans, Staphylococcus xylosus, and Staphylococcus saprophyticus revealed heterogeneous fingerprints and each (sub)species was distributed over several clusters. However, representatives of the remaining Staphylococcus spp. were clearly separated in single (sub)species-specific clusters. These results showed rep-PCR with the (GTG)5 primer as a fast and reliable method applicable for differentiation and straightforward identification of majority of Staphylococcus spp.

Links

• GA310/09/0459, research and development project: Name: Úloha bakteriofágů v horizontálním přenosu genů virulence a rezistence u Staphylococcus aureus

Investor: Czech Science Foundation, Role of bacteriophages in horizontal transfer of virulence and drug resistance genes in Staphylococcus aureus

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

• MSM0021622416, plan (intention): Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time