Acinetobacter bereziniae sp nov and Acinetobacter guillouiae sp
nov., to accommodate Acinetobacter genomic species 10 and 11,
respectively

J 2010

Acinetobacter bereziniae sp nov and Acinetobacter guillouiae sp nov., to accommodate Acinetobacter genomic species 10 and 11, respectively

NEMEC, Alexandr, Martin MUSÍLEK, Ondrej ŠEDO, Thierry DE BAERE, Martina MAIXNEROVÁ et. al.

Basic information

Original name

Acinetobacter bereziniae sp nov and Acinetobacter guillouiae sp nov., to accommodate Acinetobacter genomic species 10 and 11, respectively

Name in Czech

Acinetobacter bereziniae sp nov and Acinetobacter guillouiae sp nov odpovídající Acinetobacter genomic species 10 and 11

Authors

NEMEC, Alexandr (203 Czech Republic, guarantor), Martin MUSÍLEK (203 Czech Republic), Ondrej ŠEDO (203 Czech Republic, belonging to the institution), Thierry DE BAERE (56 Belgium), Martina MAIXNEROVÁ (203 Czech Republic), Tanny J. K. VAN DER REIJDEN (528 Netherlands), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution), Mario VANEECHOUTTE (56 Belgium) and Lenie DIJKSHOORN (528 Netherlands)

Edition

International Journal of Systematic and Evolutionary Microbiology, Reading, United Kingdom, Society for General Microbiology, 2010, 1466-5026

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.930

RIV identification code

RIV/00216224:14310/10:00051573

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1099/ijs.0.013656-0

UT WoS

000277733300033

Keywords in English

HUMAN CLINICAL SPECIMENS; GENUS ACINETOBACTER; IDENTIFICATION; BAUMANNII; PCR;

Abstract

ORIG CZ

V originále

Acinetobacter genospecies (genomic species) 10 and 11 were described by Bouvet and Grimont in 1986 on the basis of DNA DNA reassociation studies and comprehensive phenotypic analysis. In the present study, the names Acinetobacter bereziniae sp. nov. and Acinetobacter guillouiae sp. nov., respectively, are proposed for these genomic species based on the congruence of results of polyphasic analysis of 33 strains (16 and 17 strains of genomic species 10 and 11, respectively). All strains were investigated by selective restriction fragment amplification (i.e. AFLP) analysis rpoB sequence analysis, amplified rDNA restriction analysis and tDNA intergenic length polymorphism analysis, and their nutritional and physiological properties were determined. Subsets of the strains were studied by 16S rRNA gene sequence analysis and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS or had been classified previously by DNA DNA reassociation. Results indicate that A. bereziniae and A. guillouiae represent two phenetically and phylogenetically distinct groups within the genus Acinetobacter. Based on the comparative analysis of housekeeping genes (16S rRNA and rpoB genes), these species together represent a monophyletic branch within the genus. Despite their overall phenotypic similarity, the ability to oxidize o-glucose and to grow at 38 C can be used in the presumptive differentiation of these two species from each other: with the exception of three strains that were positive for only one test, A. bereziniae strains were positive for both tests, whereas A. guillouiae strains were negative in these tests. The strains of A. bereziniae originated mainly from human clinical specimens, whereas A. guillouiae strains were isolated from different environmental sources in addition to human specimens. The type strain of A. bereziniae sp. nov. is LMG 1003(T) (=CIP 70.12(T) =ATCC 17924(T)) and that of A. guillouiae sp. nov. is LMG 988(T) (=CIP 63.46(T) =ATCC 11171(T) = CC U G 2491(T)).

In Czech

Genomické specie Acinetobakter 10 a 11 byly popsány Bouvetem a Grimontem v roce 1986 na bázi DNA-DNA reasociace a fenotypu. V této práci je 33 kmenů těchto genomických specií charakterizováno AFLP, rpoB, rDNA restrikční analýzou, tDNA analýzou, 16S rRNA sekvenováním a MALDI-TOF MS. Výsledky naznačují, že se jedná o geneticky odlišitelné druhy, pro které jsou stanoveny názvy A. bereziniae a A. guillouiae.

Links

MSM0021622415, plan (intention)

Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

Citovat

NEMEC, Alexandr, Martin MUSÍLEK, Ondrej ŠEDO, Thierry DE BAERE, Martina MAIXNEROVÁ, Tanny J. K. VAN DER REIJDEN, Zbyněk ZDRÁHAL, Mario VANEECHOUTTE and Lenie DIJKSHOORN. Acinetobacter bereziniae sp nov and Acinetobacter guillouiae sp nov., to accommodate Acinetobacter genomic species 10 and 11, respectively. International Journal of Systematic and Evolutionary Microbiology. Reading, United Kingdom: Society for General Microbiology, 2010, vol. 60, No 4, p. 896-903. ISSN 1466-5026. Available from: https://dx.doi.org/10.1099/ijs.0.013656-0.

@article{911649,
   author = {Nemec, Alexandr and Musílek, Martin and Šedo, Ondrej and De Baere, Thierry and Maixnerová, Martina and van der Reijden, Tanny J. K. and Zdráhal, Zbyněk and Vaneechoutte, Mario and Dijkshoorn, Lenie},
   article_location = {Reading, United Kingdom},
   article_number = {4},
   doi = {http://dx.doi.org/10.1099/ijs.0.013656-0},
   keywords = {HUMAN CLINICAL SPECIMENS; GENUS ACINETOBACTER; IDENTIFICATION; BAUMANNII; PCR;},
   language = {eng},
   issn = {1466-5026},
   journal = {International Journal of Systematic and Evolutionary Microbiology},
   title = {Acinetobacter bereziniae sp nov and Acinetobacter guillouiae sp nov., to accommodate Acinetobacter genomic species 10 and 11, respectively},
   volume = {60},
   year = {2010}
}

TY  - JOUR
ID  - 911649
AU  - Nemec, Alexandr - Musílek, Martin - Šedo, Ondrej - De Baere, Thierry - Maixnerová, Martina - van der Reijden, Tanny J. K. - Zdráhal, Zbyněk - Vaneechoutte, Mario - Dijkshoorn, Lenie
PY  - 2010
TI  - Acinetobacter bereziniae sp nov and Acinetobacter guillouiae sp nov., to accommodate Acinetobacter genomic species 10 and 11, respectively
JF  - International Journal of Systematic and Evolutionary Microbiology
VL  - 60
IS  - 4
SP  - 896-903
EP  - 896-903
PB  - Society for General Microbiology
SN  - 14665026
KW  - HUMAN CLINICAL SPECIMENS
KW  - GENUS ACINETOBACTER
KW  - IDENTIFICATION
KW  - BAUMANNII
KW  - PCR;
N2  - Acinetobacter genospecies (genomic species) 10 and 11 were described by Bouvet and Grimont in 1986 on the basis of DNA DNA reassociation studies and comprehensive phenotypic analysis. In the present study, the names Acinetobacter bereziniae sp. nov. and Acinetobacter guillouiae sp. nov., respectively, are proposed for these genomic species based on the congruence of results of polyphasic analysis of 33 strains (16 and 17 strains of genomic species 10 and 11, respectively). All strains were investigated by selective restriction fragment amplification (i.e. AFLP) analysis rpoB sequence analysis, amplified rDNA restriction analysis and tDNA intergenic length polymorphism analysis, and their nutritional and physiological properties were determined. Subsets of the strains were studied by 16S rRNA gene sequence analysis and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS or had been classified previously by DNA DNA reassociation. Results indicate that A. bereziniae and A. guillouiae represent two phenetically and phylogenetically distinct groups within the genus Acinetobacter. Based on the comparative analysis of housekeeping genes (16S rRNA and rpoB genes), these species together represent a monophyletic branch within the genus. Despite their overall phenotypic similarity, the ability to oxidize o-glucose and to grow at 38 C can be used in the presumptive differentiation of these two species from each other: with the exception of three strains that were positive for only one test, A. bereziniae strains were positive for both tests, whereas A. guillouiae strains were negative in these tests. The strains of A. bereziniae originated mainly from human clinical specimens, whereas A. guillouiae strains were isolated from different environmental sources in addition to human specimens. The type strain of A. bereziniae sp. nov. is LMG 1003(T) (=CIP 70.12(T) =ATCC 17924(T)) and that of A. guillouiae sp. nov. is LMG 988(T) (=CIP 63.46(T) =ATCC 11171(T) = CC U G 2491(T)).
ER  -

NEMEC, Alexandr, Martin MUSÍLEK, Ondrej ŠEDO, Thierry DE BAERE, Martina MAIXNEROVÁ, Tanny J. K. VAN DER REIJDEN, Zbyněk ZDRÁHAL, Mario VANEECHOUTTE and Lenie DIJKSHOORN. Acinetobacter bereziniae sp nov and Acinetobacter guillouiae sp nov., to accommodate Acinetobacter genomic species 10 and 11, respectively. \textit{International Journal of Systematic and Evolutionary Microbiology}. Reading, United Kingdom: Society for General Microbiology, 2010, vol.~60, No~4, p.~896-903. ISSN~1466-5026. Available from: https://dx.doi.org/10.1099/ijs.0.013656-0.

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