ŘEMÍNEK, Roman, Aleš MÁDR and Zdeněk GLATZ. Monitoring of Cytochrome P450 2C9 isoform reaction with Diclofenac by In-Capillary System based on Transverse Diffusion of Laminar Flow Profiles. In Book of Abtracts 16th Latin-American Symposium LACE 2010. první. Florianopolis Brazílie: prof. Emanuel Carrilho, 2010, p. 138-138.
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Basic information
Original name Monitoring of Cytochrome P450 2C9 isoform reaction with Diclofenac by In-Capillary System based on Transverse Diffusion of Laminar Flow Profiles
Name in Czech Monitorování reakce CYP2C9 s dikclofenacem v kapiláře
Name (in English) Monitoring of Cytochrome P450 2C9 isoform reaction with Diclofenac by In-Capillary System based on Transverse Diffusion of Laminar Flow Profiles
Authors ŘEMÍNEK, Roman, Aleš MÁDR and Zdeněk GLATZ.
Edition první. Florianopolis Brazílie, Book of Abtracts 16th Latin-American Symposium LACE 2010, p. 138-138, 1 pp. 2010.
Publisher prof. Emanuel Carrilho
Other information
Type of outcome Proceedings paper
Confidentiality degree is not subject to a state or trade secret
Organization unit Faculty of Science
Keywords (in Czech) EMMA; cytochromy P450; metabolismus léčiv; diclofenac
Keywords in English EMMA; cytochromes P450; drug metabolism; diclofenac
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Zdeněk Glatz, CSc., učo 1865. Changed: 13/12/2010 15:45.
Abstract
In this study a method for cytochrome P450 2C9 (CYP2C9) reaction with diclofenac as a probe substrate was developed. 30 min incubation time and sandwich layout, where a plug of CYP2C9 is surrounded by two plugs of diclofenac and NADPH mixture, were selected as appropriate incubation conditions for final method within the optimizing. Although bare silica capillary was used for performing the analyses of samples consisting of enzyme preparations, strong rinsing procedure with 1 M H3PO4 and 1 M NaOH applied between following runs provides good method's repeatability with RSD values of 1.52 % and 4.83 % (n = 6) for migration times and peak areas of 4'-hydroxydiclofenac, respectively. Since the methodology of TDLFP allows changing the incubation mixture composition in simple way, the introduced method represents a promising tool for in-capillary inhibition studies of CYP2C9.
Abstract (in Czech)
Byla vypracována metoda pro monitorováni reakce CYP2C9 s dikclofenacem v kapiláře
Abstract (in English)
In this study a method for cytochrome P450 2C9 (CYP2C9) reaction with diclofenac as a probe substrate was developed. 30 min incubation time and sandwich layout, where a plug of CYP2C9 is surrounded by two plugs of diclofenac and NADPH mixture, were selected as appropriate incubation conditions for final method within the optimizing. Although bare silica capillary was used for performing the analyses of samples consisting of enzyme preparations, strong rinsing procedure with 1 M H3PO4 and 1 M NaOH applied between following runs provides good method's repeatability with RSD values of 1.52 % and 4.83 % (n = 6) for migration times and peak areas of 4'-hydroxydiclofenac, respectively. Since the methodology of TDLFP allows changing the incubation mixture composition in simple way, the introduced method represents a promising tool for in-capillary inhibition studies of CYP2C9.
Links
GAP206/10/0057, research and development projectName: Miniaturizovaný systém pro "on-line" studie metabolismu léčiv založený na kapilární elektroforéze
Investor: Czech Science Foundation, Miniaturized on-line drug metabolism system based on capillary electrophoresis
LC06023, research and development projectName: Integrované bioanalytické technologie pro mikroanalýzy a diagnostiku s využitím LIF a hmotnostní spektrometrie
Investor: Ministry of Education, Youth and Sports of the CR
MSM0021622413, plan (intention)Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment
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