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@inproceedings{913154, author = {Fojtová, Miloslava and Horáková, Petra and Pivoňková, Hana and Vychodilová, Zdenka and Fojta, Miroslav}, address = {Ústí nad Labem}, booktitle = {XXX. Moderní Elektrochemické Metody}, keywords = {OSMIUM-TETROXIDE COMPLEXES; NUCLEOSIDE TRIPHOSPHATES; ETHIDIUM-BROMIDE; HYBRIDIZATION; ASSAY; BASE}, language = {eng}, location = {Ústí nad Labem}, isbn = {978-80-254-6710-7}, publisher = {BEST servis Ústí nad Labem}, title = {Utilization of electrochemical activity of 7-deazaguanine in the monitoring of PCR amplification of G-rich DNA Strands.}, year = {2010} }
TY - JOUR ID - 913154 AU - Fojtová, Miloslava - Horáková, Petra - Pivoňková, Hana - Vychodilová, Zdenka - Fojta, Miroslav PY - 2010 TI - Utilization of electrochemical activity of 7-deazaguanine in the monitoring of PCR amplification of G-rich DNA Strands. PB - BEST servis Ústí nad Labem CY - Ústí nad Labem SN - 9788025467107 KW - OSMIUM-TETROXIDE COMPLEXES KW - NUCLEOSIDE TRIPHOSPHATES KW - ETHIDIUM-BROMIDE KW - HYBRIDIZATION KW - ASSAY KW - BASE N2 - 7-deazaguanine, guanine analogue, is able to be sequence specifically incorporated into DNA. Modified DNA fragments can not be detected by standard techniques (gel electrophoresis, quantitative PCR) because of quenching of fluorescence of commonly used dyes. Electrochemical detection provides 7-deazaguanine-specific signal suitable for the quantification of PCR product and determination of the extent of DNA modification. ER -
FOJTOVÁ, Miloslava, Petra HORÁKOVÁ, Hana PIVOŇKOVÁ, Zdenka VYCHODILOVÁ a Miroslav FOJTA. Utilization of electrochemical activity of 7-deazaguanine in the monitoring of PCR amplification of G-rich DNA Strands. Online. In \textit{XXX. Moderní Elektrochemické Metody}. Ústí nad Labem: BEST servis Ústí nad Labem, 2010. 5 s. ISBN~978-80-254-6710-7. [citováno 2024-04-24]
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