Assessment of adenosine triphosphate-binding cassette subfamily
B member 1 (ABCB1) mRNA expression in patients with de novo
chronic myelogenous leukemia: the role of different cell types.

J 2011

Assessment of adenosine triphosphate-binding cassette subfamily B member 1 (ABCB1) mRNA expression in patients with de novo chronic myelogenous leukemia: the role of different cell types.

RÁČIL, Zdeněk, Filip RÁZGA, Kateřina MACHOVÁ POLÁKOVÁ, Lucie BUREŠOVÁ, Václava POLÍVKOVÁ et. al.

Basic information

Original name

Assessment of adenosine triphosphate-binding cassette subfamily B member 1 (ABCB1) mRNA expression in patients with de novo chronic myelogenous leukemia: the role of different cell types.

Authors

RÁČIL, Zdeněk (203 Czech Republic, guarantor, belonging to the institution), Filip RÁZGA (703 Slovakia, belonging to the institution), Kateřina MACHOVÁ POLÁKOVÁ (203 Czech Republic), Lucie BUREŠOVÁ (203 Czech Republic, belonging to the institution), Václava POLÍVKOVÁ (203 Czech Republic), Dana DVOŘÁKOVÁ (203 Czech Republic, belonging to the institution), Daniela ŽÁČKOVÁ (203 Czech Republic, belonging to the institution), Hana KLAMOVÁ (203 Czech Republic), Petr CETKOVSKÝ (203 Czech Republic) and Jiří MAYER (203 Czech Republic, belonging to the institution)

Edition

Leukemia & Lymphoma, London, Informa UK, Ltd. 2011, 1042-8194

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.580

RIV identification code

RIV/00216224:14740/11:00051592

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.3109/10428194.2010.533220

UT WoS

000287089100027

Keywords (in Czech)

mRNA expression; CML

Keywords in English

mRNA exprese; CML

Abstract

V originále

In this study, we investigated differences in ABCB1 mRNA expression measured in peripheral blood (PB) leukocytes (LEU) as well as polymorphonuclear (PMNCs) and mononuclear cells (MNCs) of PB LEU obtained from healthy volunteers and patients with de novo CML. In addition, we analyzed the relationship between the percentage of individual cells that comprise the total LEU count and ABCB1 mRNA expression assessed from the total LEU. We have shown that ABCB1 mRNA transcript levels are significantly higher in PB MNCs than in PB PMNCs and are lower in patients with de novo CML compared to healthy individuals. Moreover, we have demonstrated the importance of the cell composition of analyzed samples in ABCB1 mRNA expression analysis.

Links

MSM0021622430, plan (intention)

Name: Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie

Investor: Ministry of Education, Youth and Sports of the CR, Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies

Citovat

RÁČIL, Zdeněk, Filip RÁZGA, Kateřina MACHOVÁ POLÁKOVÁ, Lucie BUREŠOVÁ, Václava POLÍVKOVÁ, Dana DVOŘÁKOVÁ, Daniela ŽÁČKOVÁ, Hana KLAMOVÁ, Petr CETKOVSKÝ and Jiří MAYER. Assessment of adenosine triphosphate-binding cassette subfamily B member 1 (ABCB1) mRNA expression in patients with de novo chronic myelogenous leukemia: the role of different cell types. Leukemia & Lymphoma. London: Informa UK, Ltd., 2011, vol. 52, No 2, p. 331-334. ISSN 1042-8194. Available from: https://dx.doi.org/10.3109/10428194.2010.533220.

@article{914486,
   author = {Ráčil, Zdeněk and Rázga, Filip and Machová Poláková, Kateřina and Burešová, Lucie and Polívková, Václava and Dvořáková, Dana and Žáčková, Daniela and Klamová, Hana and Cetkovský, Petr and Mayer, Jiří},
   article_location = {London},
   article_number = {2},
   doi = {http://dx.doi.org/10.3109/10428194.2010.533220},
   keywords = {mRNA exprese; CML},
   language = {eng},
   issn = {1042-8194},
   journal = {Leukemia & Lymphoma},
   title = {Assessment of adenosine triphosphate-binding cassette subfamily B member 1 (ABCB1) mRNA expression in patients with de novo chronic myelogenous leukemia: the role of different cell types.},
   volume = {52},
   year = {2011}
}

TY  - JOUR
ID  - 914486
AU  - Ráčil, Zdeněk - Rázga, Filip - Machová Poláková, Kateřina - Burešová, Lucie - Polívková, Václava - Dvořáková, Dana - Žáčková, Daniela - Klamová, Hana - Cetkovský, Petr - Mayer, Jiří
PY  - 2011
TI  - Assessment of adenosine triphosphate-binding cassette subfamily B member 1 (ABCB1) mRNA expression in patients with de novo chronic myelogenous leukemia: the role of different cell types.
JF  - Leukemia & Lymphoma
VL  - 52
IS  - 2
SP  - 331-334
EP  - 331-334
PB  - Informa UK, Ltd.
SN  - 10428194
KW  - mRNA exprese
KW  - CML
N2  - In this study, we investigated differences in ABCB1 mRNA expression measured in peripheral blood (PB) leukocytes (LEU) as well as polymorphonuclear (PMNCs) and mononuclear cells (MNCs) of PB LEU obtained from healthy volunteers and patients with de novo CML. In addition, we analyzed the relationship between the percentage of individual cells that comprise the total LEU count and ABCB1 mRNA expression assessed from the total LEU. We have shown that ABCB1 mRNA transcript levels are significantly higher in PB MNCs than in PB PMNCs and are lower in patients with de novo CML compared to healthy individuals. Moreover, we have demonstrated the importance of the cell composition of analyzed samples in ABCB1 mRNA expression analysis.
ER  -

RÁČIL, Zdeněk, Filip RÁZGA, Kateřina MACHOVÁ POLÁKOVÁ, Lucie BUREŠOVÁ, Václava POLÍVKOVÁ, Dana DVOŘÁKOVÁ, Daniela ŽÁČKOVÁ, Hana KLAMOVÁ, Petr CETKOVSKÝ and Jiří MAYER. Assessment of adenosine triphosphate-binding cassette subfamily B member 1 (ABCB1) mRNA expression in patients with de novo chronic myelogenous leukemia: the role of different cell types. \textit{Leukemia \&{} Lymphoma}. London: Informa UK, Ltd., 2011, vol.~52, No~2, p.~331-334. ISSN~1042-8194. Available from: https://dx.doi.org/10.3109/10428194.2010.533220.

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