KUNOVÁ, Michaela, Kamil MATULKA, Lívia EISELLEOVÁ, Petra TRČKOVÁ, Aleš HAMPL and Petr DVOŘÁK. Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells. Reproductive BioMedicine Online. 2010, vol. 21, No 5, p. 76-86. ISSN 1472-6483.
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Basic information
Original name Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells
Authors KUNOVÁ, Michaela (203 Czech Republic, guarantor, belonging to the institution), Kamil MATULKA (203 Czech Republic, belonging to the institution), Lívia EISELLEOVÁ (703 Slovakia, belonging to the institution), Petra TRČKOVÁ (203 Czech Republic, belonging to the institution), Aleš HAMPL (203 Czech Republic) and Petr DVOŘÁK (203 Czech Republic).
Edition Reproductive BioMedicine Online, 2010, 1472-6483.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.285
RIV identification code RIV/00216224:14110/10:00046015
Organization unit Faculty of Medicine
UT WoS 000284797000016
Keywords in English animal protein-free culture; high-density culture; human embryonic stem cells; humanized culture medium; plant-derived serum supplement; VegetaCell
Changed by Changed by: Ing. Mgr. Věra Pospíšilíková, učo 9005. Changed: 1/8/2013 11:33.
Abstract
For human embryonic stem cells (ESC) to be used in cell replacement therapies, they must be grown under good manufacturing conditions in a chemically defined medium that lacks animal proteins. This study examined the ability of a newly designed medium containing the plant-derived serum replacement VegetaCell and other reagents of human origin to support undifferentiated growth and pluripotency of human ESC. This medium was tested in several culture systems, using human fibroblasts as a feeder layer or Matrigel in a feeder-free culture. Even under the most stringent feeder-free conditions without conditioned medium, human ESC exhibited an undifferentiated morphology, expressed markers of undifferentiated cells, demonstrated high alkaline phosphatase activity and multilineage differentiation and retained a normal karyotype. Compared with human ESC grown in standard culture conditions, human ESC maintained in humanized VegetaCell medium show longer cell cycles and decreased cell death. The availability of an animal protein-free medium supplemented with the low-cost VegetaCell reagent expands the repertoire of media for culturing human ESC as well as induced pluripotent stem cells for drug testing and cell replacement therapy. For human embryonic stem cells (ESC) to be used in cell replacement therapies, they must be grown under good manufacturing conditions in a chemically defined medium that lacks animal proteins. This study examined the ability of a newly designed medium containing the plant-derived serum replacement VegetaCell and other reagents of human origin to support undifferentiated growth and pluripotency of human ESC. This medium was tested in several culture systems, using human fibroblasts as a feeder layer or Matrigel in a feeder-free culture. Even under the most stringent feeder-free conditions without conditioned medium, human ESC exhibited an undifferentiated morphology, expressed markers of undifferentiated cells, demonstrated high alkaline phosphatase activity and multilineage differentiation and retained a normal karyotype. Compared with human ESC grown in standard culture conditions, human ESC maintained in humanized VegetaCell medium show longer cell cycles and decreased cell death. The availability of an animal protein-free medium supplemented with the low-cost VegetaCell reagent expands the repertoire of media for culturing human ESC as well as induced pluripotent stem cells for drug testing and cell replacement therapy.
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