J 2010

Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells

KUNOVÁ, Michaela, Kamil MATULKA, Lívia EISELLEOVÁ, Petra TRČKOVÁ, Aleš HAMPL et. al.

Basic information

Original name

Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells

Authors

KUNOVÁ, Michaela (203 Czech Republic, guarantor, belonging to the institution), Kamil MATULKA (203 Czech Republic, belonging to the institution), Lívia EISELLEOVÁ (703 Slovakia, belonging to the institution), Petra TRČKOVÁ (203 Czech Republic, belonging to the institution), Aleš HAMPL (203 Czech Republic) and Petr DVOŘÁK (203 Czech Republic)

Edition

Reproductive BioMedicine Online, 2010, 1472-6483

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.285

RIV identification code

RIV/00216224:14110/10:00046015

Organization unit

Faculty of Medicine

UT WoS

000284797000016

Keywords in English

animal protein-free culture; high-density culture; human embryonic stem cells; humanized culture medium; plant-derived serum supplement; VegetaCell
Změněno: 1/8/2013 11:33, Ing. Mgr. Věra Pospíšilíková

Abstract

V originále

For human embryonic stem cells (ESC) to be used in cell replacement therapies, they must be grown under good manufacturing conditions in a chemically defined medium that lacks animal proteins. This study examined the ability of a newly designed medium containing the plant-derived serum replacement VegetaCell and other reagents of human origin to support undifferentiated growth and pluripotency of human ESC. This medium was tested in several culture systems, using human fibroblasts as a feeder layer or Matrigel in a feeder-free culture. Even under the most stringent feeder-free conditions without conditioned medium, human ESC exhibited an undifferentiated morphology, expressed markers of undifferentiated cells, demonstrated high alkaline phosphatase activity and multilineage differentiation and retained a normal karyotype. Compared with human ESC grown in standard culture conditions, human ESC maintained in humanized VegetaCell medium show longer cell cycles and decreased cell death. The availability of an animal protein-free medium supplemented with the low-cost VegetaCell reagent expands the repertoire of media for culturing human ESC as well as induced pluripotent stem cells for drug testing and cell replacement therapy. For human embryonic stem cells (ESC) to be used in cell replacement therapies, they must be grown under good manufacturing conditions in a chemically defined medium that lacks animal proteins. This study examined the ability of a newly designed medium containing the plant-derived serum replacement VegetaCell and other reagents of human origin to support undifferentiated growth and pluripotency of human ESC. This medium was tested in several culture systems, using human fibroblasts as a feeder layer or Matrigel in a feeder-free culture. Even under the most stringent feeder-free conditions without conditioned medium, human ESC exhibited an undifferentiated morphology, expressed markers of undifferentiated cells, demonstrated high alkaline phosphatase activity and multilineage differentiation and retained a normal karyotype. Compared with human ESC grown in standard culture conditions, human ESC maintained in humanized VegetaCell medium show longer cell cycles and decreased cell death. The availability of an animal protein-free medium supplemented with the low-cost VegetaCell reagent expands the repertoire of media for culturing human ESC as well as induced pluripotent stem cells for drug testing and cell replacement therapy.