a 2010

Disruption of retinoid signalling and differentiation process by PAHs and N-PAHs in vitro

BENÍŠEK, Martin, Luděk BLÁHA, Klára HILSCHEROVÁ and Petra KUBINCOVÁ

Basic information

Original name

Disruption of retinoid signalling and differentiation process by PAHs and N-PAHs in vitro

Authors

BENÍŠEK, Martin (203 Czech Republic, guarantor, belonging to the institution), Luděk BLÁHA (203 Czech Republic, belonging to the institution), Klára HILSCHEROVÁ (203 Czech Republic, belonging to the institution) and Petra KUBINCOVÁ (203 Czech Republic, belonging to the institution)

Edition

SETAC Europe, 20th Annual Meeting, Seville, Spain, 2010

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

30304 Public and environmental health

Country of publisher

Belgium

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/10:00046568

Organization unit

Faculty of Science

Keywords in English

retinoids; differentiation; PAHs; N-PAHs

Tags

International impact
Změněno: 10/1/2011 14:17, Mgr. Martin Beníšek, Ph.D.

Abstract

V originále

Polycyclic aromatic hydrocarbons (PAHs) are similar to their N-heterocyclic analogs (N-PAHs) important and widespread environmental contaminants generated especially by the incomplete combustion of organic materials. Many of them are known carcinogens, mutagens and teratogens. Teratogenicity of PAHs and N-PAHs could be caused by disruption of retinoid signaling by these compounds, as retinoid levels are crucial for differentiation process and normal embryonic development. In this study the interference of PAHs and N-PAHs with retinoid signaling system was tested using P19/A15 cell line (Approximately one half of tested compounds downregulated RA activity after 6h exposure with lower or no downregulation after 24 h. After 24 h exposure most of three, four and five ring PAHs and N-PAHs increased RA activity. Finally, P19 cells were exposed to selected active compounds either alone or in co-exposure with RA and effects on differentiation process (analysis of Oct-4 protein levels modulation) were assessed.

Links

2B08036, research and development project
Name: Nové molekulárně biologické a biochemické metody pro monitoring estrogenů a dalších chemických endokrinních disruptorů (Acronym: ENVISCREEN)
Investor: Ministry of Education, Youth and Sports of the CR, New molecular biology and biochemical methods to monitor estrogens and other endocrine disruptors in the environment of the C.R.