Electrophysiological Effects of Voltage Sensitive Dye di-4-ANNEPS in Rabbit and Guinea Pig Isolated Heart.

FIALOVÁ, Kateřina, Jana KOLÁŘOVÁ, Jiří SEKORA, Milan TANNENBERG, Marie NOVÁKOVÁ and Ivo PROVAZNÍK. Electrophysiological Effects of Voltage Sensitive Dye di-4-ANNEPS in Rabbit and Guinea Pig Isolated Heart. In Biosignal 2010. 2010. ISBN 978-80-214-4105-7.

Basic information

• Original name: Electrophysiological Effects of Voltage Sensitive Dye di-4-ANNEPS in Rabbit and Guinea Pig Isolated Heart.
• Name in Czech: Elektrofyziologické účinky napěťově citlivého barviva di-4-ANEPPS studované na modelu izolovaného srdce králíka a morčete.
• Authors: FIALOVÁ, Kateřina, Jana KOLÁŘOVÁ, Jiří SEKORA, Milan TANNENBERG, Marie NOVÁKOVÁ and Ivo PROVAZNÍK.
• Edition: Biosignal 2010, 2010.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 30105 Physiology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Medicine
• ISBN: 978-80-214-4105-7
• Keywords (in Czech): napěťově citlivé barvivo; izolované srdce
• Keywords in English: voltage sensitive dye; isolated heart

Abstract

Mapping of cardiac electrical activity using voltage-sensitive dyes (VSDs) is considered to be a valuable method for electrophysiological studies. VSDs undergo changes in their electronic structure, and consequently their fluorescence spectra, in response to changes in the surrounding electric field and therefore may be used for recording of monophasic action potentials (MAPs). Staining of the heart tissue with the dye should not result in pharmacological or toxic effects. VSD di-4-ANEPPS is currently used in our laboratory for recording of MAPs in isolated hearts. This paper presents continuation of our previous study focused on examination of electrophysiological effects of this dye on rabbit and guinea pig isolated hearts. Electrograms, recorded during staining with the dye and washout from it, were compared and the types and frequency of dysrhythmias evaluated.

Links

• GA102/07/1473, research and development project: Name: Optické metody registrace elektrických potenciálů a koncentrace vápníku v srdci s laserovou stabilizací

Investor: Czech Science Foundation

• MSM0021622402, plan (intention): Name: Časná diagnostika a léčba kardiovaskulárních chorob

Investor: Ministry of Education, Youth and Sports of the CR, Early diagnostics and treatment of cardiovascular diseases