Polyaniline Sulfonate/Cytochrome c Multilayer Electrodes: Polyelectrolytes Properties and Assembly Characteristics

SARAULI, Fred Lisdat David, Johannes TANNE, Chenggang XU, Burkhard SCHULZ and Libuše TRNKOVÁ. Polyaniline Sulfonate/Cytochrome c Multilayer Electrodes: Polyelectrolytes Properties and Assembly Characteristics. In 61st Annual Meeting of the International Society of Electrochemistry, the book of abstracts. 2010. ISBN 978-80-7375-396-2.

Basic information

• Original name: Polyaniline Sulfonate/Cytochrome c Multilayer Electrodes: Polyelectrolytes Properties and Assembly Characteristics
• Authors: SARAULI, Fred Lisdat David (276 Germany), Johannes TANNE (276 Germany), Chenggang XU (276 Germany), Burkhard SCHULZ (276 Germany) and Libuše TRNKOVÁ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: 61st Annual Meeting of the International Society of Electrochemistry, the book of abstracts, 2010.

Other information

• Original language: English
• Type of outcome: Presentations at conferences
• Field of Study: 10403 Physical chemistry
• Country of publisher: France
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/10:00051669
• Organization unit: Faculty of Science
• ISBN: 978-80-7375-396-2
• Keywords (in Czech): QCM;CV;PASA
• Keywords in English: QCM;CV;PASA
• Tags: AKb, rivok

Abstract

Among the variety of different polyelectrolytes, sulfonated polyaniline (PASA) is found to be suitable for the construction of electroactive cyt c-containing multilayers. On the surface of gold electrodes subsequently coated with up to 16 alternating layers of cyt c and PASA the immobilized protein is fully electroactive, retains its native structure and can be successfully applied as a sensor for superoxide [1]. Here we present a study of the polyelectrolyte influence on the multilayer formation and redox properties of the assemblies formed. Two different sulfonated polyanilines (PASA1 [2] and PASA2 [3]) have been chemically synthesized, the assembly build-up has been investigated by Quartz Crystal Microbalance (QCM), and characterization of these systems on electrodes has been performed by Cyclic Voltammetry (CV). The chemically synthesized PASA versions differ in the grade of sulfonation, chain length, overall charge and the presence of an additional methoxy group on the aniline ring. The assembly of both polymers with cyt c is successful, which follows from the results of QCM measurements. Electrochemical studies show an increase in amount of electroactive species on the electrode surface with increasing number of layers in both cases. However, in the case of PASA2, which is shorter than PASA1 and has an additional methoxy group in its structure, higher electroactivity of the polyaniline itself has been observed. This may cause side reactions when the system would be applied in sensorics. Multilayer assemblies with PASA1, in contrast, show a behavior dominated by the redox activity of cyt c , which makes them promising for sensor approaches, e.g for superoxide detection. The study demonstrates that several requirements have to be fulfilled in order to obtain functional protein assemblies on electrodes.

Links

• LC06035, research and development project: Name: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.

Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)